The ZR Plasmid Miniprep Classic kits from Zymo Research enable the efficient isolation of endotoxin-free plasmid DNA from E. coli in minutes using a traditional 3-buffer (P1, P2, P3) procedure.
Benefits of using ZR Plasmid Miniprep Classic kits
• High quality, endotoxin-free plasmid DNA isolated
• Short protocol
• Low 30 µl elution volume
• Zero buffer retention
• Easy visualisation of complete lysis and neutralisation using coloured buffers
• Suitable for all sensitive downstream applications including restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation and transfection
Complete bacterial cell lysis is visualised using the unique coloured buffer system
Purified plasmid DNA is well suited for downstream applications including restriction endonuclease digestion and DNA sequencing
Left: Endonuclease digestion of three different DNA plasmids prepared using the ZR Plasmid Miniprep-Classic (performed in triplicate). M: ZR 1 kb DNA Marker. Right: DNA sequencing chromatogram of plasmid DNA prepared using the ZR Plasmid Miniprep-Classic.
Material available for download
ZR Plasmid Miniprep-Classic protocol
ZR Plasmid Miniprep-Classic datasheet
White paper: Reprograming the replisome of a semi-synthetic organism for the expansion of the genetic alphabet