Recombinant AAV and lentiviral production: TransIT-VirusGEN

TransIT-VirusGEN® from Mirus Bio is a high-performance transfection reagent for the reliable and efficient delivery of packaging and transfer vectors to adherent or suspension HEK293 cell types. With a streamlined recombinant adeno-associated virus (AAV) and lentivirus production workflow and highly efficient DNA delivery, TransIT-VirusGEN can be used with a variety of cell culture systems.

Benefits of using TransIT-VirusGEN
• Consistent high virus titre production
• Compatible with adherent or suspension HEK293 cells
• Can be scaled efficiently
• Streamlined virus generation workflow
• Compatible with different virus & cell culture systems

Read more about the preclinical grade alternative, TransIT-VirusGEN SELECT, which is ideal large-scale production of viral vectors for clinical trials.

TransIT-VirusGEN outperforms competitor reagents in suspension and adherent AAV cell cultures

TransIT-VirusGEN® Outperforms Competitor Reagents in Suspension & Adherent AAV Cell Cultures.

Suspension (A) or adherent cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio) with the following reagents: TransIT-VirusGEN (2:1, vol:wt), Lipofectamine® 2000 (3:1), Lipofectamine® 3000 (3:3:1) or 25 kDa PEI (4:1). Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction using guava easyCyte™ 5HT flow cytometer. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.

TransIT-VirusGEN enables broad scalability (AAV)

TransIT-VirusGEN® Enables Broad Scalability (AAV)

Suspension (A) or adherent cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio) using the TransIT-VirusGEN transfection reagent (2:1, vol:wt) at the indicated volumes per culture vessel. Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction by flow cytometry. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. Error bars represent the standard deviation of triplicate wells, flasks, or dishes.

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Material available for download
TransIT-VirusGEN protocol
TransIT-VirusGEN quick reference protocol for AAV generation
TransIT-VirusGEN SDS

Lipofectamine is a registered trademark of Life Technologies Corporation
Guava and EasyCyte are registered trademarks of Guava Technologies, Inc.

Lentivirus Production, Transfection Kit

Products

Note: product availability depends on country - see product detail page.

Details	Cat number & supplier	Size	Price
RevIT AAV Enhancer MIR 8000 · Mirus	MIR 8000 Mirus	1.5 ml for 1L of Cell Culture	£369.00 1.5 ml for 1L of Cell Culture view
RevIT AAV Enhancer MIR 8006 · Mirus	MIR 8006 Mirus	10 x 1.5ml for 10L of Cell Culture	£3688.00 10 x 1.5ml for 10L of Cell Culture view
VirusGEN AAV RevIT 1L Kit MIR 8007 · Mirus	MIR 8007 Mirus	For 1L of Culture	£1453.00 For 1L of Culture view
VirusGEN® AAV Transfection Kit with RevIT™ AAV Enhancer For 10L of Culture MIR 8008 · Mirus	MIR 8008 Mirus	1 kit	£11381.00 1 kit view
TransIT-VirusGEN Transfection Reagent MIR 6700 · Mirus	MIR 6700 Mirus	1.5 ml	£729.00 1.5 ml view
TransIT-VirusGEN Transfection Reagent MIR 6703 · Mirus	MIR 6703 Mirus	0.3 ml	£183.00 0.3 ml view
TransIT-VirusGEN Transfection Reagent MIR 6704 · Mirus	MIR 6704 Mirus	0.75 ml	£461.00 0.75 ml view
TransIT-VirusGEN Transfection Reagent MIR 6705 · Mirus	MIR 6705 Mirus	5 x 1.5 ml	£3173.00 5 x 1.5 ml view
TransIT-VirusGEN Transfection Reagent MIR 6706 · Mirus	MIR 6706 Mirus	10 x 1.5 ml	£5836.00 10 x 1.5 ml view

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