Transgenesis in one transfection - PiggyBac™

System Biosciences' transposon vector system includes a large portfolio of PiggyBac™ transposon vectors and PiggyBac™ transposase expression vectors. In just one simple transfection, achieve instant, reversible and inducible transgenesis in numerous animal species.

Benefits of using the PiggyBac™ transposon vector system
• Cost-effective
• Highly efficient nonviral gene delivery
• High transposition efficiency and activity
• Transgenic cell lines in one transfection
• Reversible transposition - no footprint
• Single or multiple integrations from a single transfection enabled
• No cargo limit - integrate 10-100kb
• Suitable for use in human, mouse and rat cells

About the PiggyBac™ transposon
The TTAA-specific transposon PiggyBac™ is a mobile genetic element that efficiently transposes between vectors and chromosomes via a "cut and paste" mechanism. During transposition, the PiggyBac™ transposase recognises transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. This feature enables genes to be easily and efficiently mobilised into target genomes.

Cut and paste integration using a single transfection

PiggyBac

The PiggyBac™ transposon vectors are available in a number of formats:
• Single Promoter - GFP, RFP and Neo
• Dual Promoter - Puro, GFP, RFP, GFP+Puro or RFP+Puro
• shRNA Vectors - CMV or EF1alpha promoters express the GFP+Puro dual markers
• The PiggyBac™ all-in-one inducible vector requires the cumate solution for induction

PiggyBac™ licensing agreements
Prior to any purchase, customers must agree to the terms of the PiggBac™ agreements set out by SBI. Please contact us to discuss your requirements.

Material available for download
PiggyBac™ Transposon Vector System Product Sheet
PiggyBac™ Transposon Vector System User Manual

PiggyBac™ citation list
 

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Cumate Solution, high concentration, 10,000x for use with PiggyBac PBQM100A-1 · System Biosciences
PBQM100A-1
System Biosciences
500 µl POA
500 µl
view
PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector PB511B-1 · System Biosciences
PB511B-1
System Biosciences
10 µg POA
10 µg
view
PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector PB513B-1 · System Biosciences
PB513B-1
System Biosciences
10 µg POA
10 µg
view
PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector PB510B-1 · System Biosciences
PB510B-1
System Biosciences
10 µg POA
10 µg
view
PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector PB512B-1 · System Biosciences
PB512B-1
System Biosciences
10 µg POA
10 µg
view
PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector PBSI505A-1 · System Biosciences
PBSI505A-1
System Biosciences
10 µg POA
10 µg
view
PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector PBSI506A-1 · System Biosciences
PBSI506A-1
System Biosciences
10 µg POA
10 µg
view
PB-SV40-Neo-EF1-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector PB400A-1 · System Biosciences
PB400A-1
System Biosciences
10 µg POA
10 µg
view
PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector PB713B-1 · System Biosciences
PB713B-1
System Biosciences
10 µg POA
10 µg
view
PB-CMV-miR-302/367-EF1-GFP iPSC Vector PB-MIR302 · System Biosciences
PB-MIR302
System Biosciences
10 µg POA
10 µg
view
PB-Cuo-miR-302/367-IRES-GFP-EF1-CymR-Puro Inducible iPSC Vector PBQM-MIR302 · System Biosciences
PBQM-MIR302
System Biosciences
10 µg POA
10 µg
view
PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector PB514B-2 · System Biosciences
PB514B-2
System Biosciences
10 µg POA
10 µg
view
PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector PB530A-2 · System Biosciences
PB530A-2
System Biosciences
10 µg POA
10 µg
view
PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector PBQM812A-1 · System Biosciences
PBQM812A-1
System Biosciences
10 µg POA
10 µg
view
PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector PB531A-2 · System Biosciences
PB531A-2
System Biosciences
10 µg POA
10 µg
view
PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector PB533A-2 · System Biosciences
PB533A-2
System Biosciences
10 µg POA
10 µg
view
Excision only piggyBac Transposase expression vector, 50 rxns PB220PA-1 · System Biosciences
PB220PA-1
System Biosciences
10 µg POA
10 µg
view