High-throughput EV isolation from serum or plasma: SmartSEC HT

SmartSEC™ HT from System Biosciences is the first kit on the market for high-throughput, plate-based extracellular vesicle isolation. Enabling isolation from up to 96 samples at a time, SmartSEC HT is based on a proprietary chromatography-based EV isolation method which combines all the benefits of size exclusion chromatography (SEC)—purity, yield, reproducibility and preservation of EV integrity—with a contaminant trapping feature that overcomes the limitations of conventional SEC for a fast and easy workflow designed for both manual and automated liquid handling instruments.

SmartSEC HT workflow

Benefits of using SmartSEC HT EV Isolation System
• Isolate EVs from 96 samples in less than one hour
• Use all the wells in a single run or run a partial plate and save wells for later use
• Load serum/plasma directly onto the plate after a brief centrifugation—no need to pre-treat plasma
• Achieve better levels of purity and yield compared to ultracentrifugation
• Isolated EVs are compatible with most downstream applications

SSmartSEC HT delivers high yields of EVs with low amounts of undesirable carry-over protein

EVs were prepared from 500 µL of serum (A) or plasma (B) using the indicated methods. For Western blot analysis, 1 µg protein equivalent from the first fraction was loaded into each lane. SmartSEC HT performs better than ultracentrifugation and a competitor’s “q” SEC column.

SmartSEC HT delivers higher yields than ultracentrifugation and a competitor’s “q” SEC column

Prepared EVs from 250 µL and 500 µL of serum and plasma using the indicated methods and determined yield using using (A) a Qubit protein assay and (B) fluorescent nanoparticle tracking analysis (fNTA). Table 1 provides a tabular summary of the plotted data values. By both EV yield methods, SmartSEC HT outperforms the other methods.

Applications
• Biomarker discovery
• Diagnostic development
• Therapeutic development

Material available for download
SmartSEC HT EV Isolation System user manual
SmartSEC HT EV Isolation System product sheet