Small RNA sequencing library preparation

The Small RNA-Seq Library Prep Kit from Lexogen generates Illumina®-compatible small RNA sequencing libraries, directly from total RNA or enriched small RNA. Optimised for low RNA content samples such as plasma, serum or urine, ready-to-sequence libraries can be prepared using as little as 50 pg to 1,000 ng RNA input in less than 5 hours. The quick and convenient magnetic bead-based purification method removes adapters dimers for even the most demanding of RNA inputs. 

Benefit of using the Small RNA-Seq Library Prep kit
• Fast and streamlined protocol with gel-free size selection in under 5 hours
• Magnetic bead-based purification method removes adapter dimers
• Optimised for low RNA content samples including plasma, serum and urine
• Compatible with RNA input as low as 50 pg to 1,000 ng
• i7 indices included allowing multiplexing of up to 96 samples
• Enables exceptional miRNA discovery
• Includes all components required to generate small RNA libraries including adapters, primers, enzyme mixes, and buffers

Detection of a high number of microRNAs with the Small RNA-Seq Library Prep kit

Total number of miRNAs detected across 4 different protocols. Dilution series (6 pg, 60 pg, and 600 pg) of purified plasma RNA used for library prep from different vendors were performed. The obtained libraries were sequenced at equal molarity at ~ 1.5 – 2M Total Raw Reads per sample. Lexogen’s Small RNA-Seq Kit showed much higher numbers of detected miRNAs at ≥ 5 Raw Reads across all concentrations tested.

Applications
• Sequencing of small RNAs, including non-coding small RNAs such as microRNA (miRNA) and small interfering RNA (siRNA)

Material available for download
Small RNA-Seq Library Prep Kit flyer
Small RNA-Seq Library Prep Kit user guide

Small RNA-Seq citations

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