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Routine cloning and library construction competent cells

The E. cloni® competent cells from Lucigen incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability. Available in chemically and electrocompetent formats in a range of high transformation efficiencies, the E.cloni cells are ideal as direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10 and XL1-Blue).

Benefits of the E. cloni range
• High yield optimised
• Directly replace standard cloning strains
• phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable
• Range of high electro competent transformation efficiencies (5 x 109 to 4 x 1010 cfu/µg)
• Range of high chemically competent transformation efficiencies (1 x 106 to 1 x 109 cfu/µg)
 

Format Name Efficiency Applications
Electrocompetent E. cloni 10G SUPREME Electrocompetent Cells > 4 x 1010 cfu/µg pUC DNA SUPREME Cells are suitable for construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.
E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 x 1010 cfu/µg pUC DNA ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA.
E. cloni 10G CLASSIC Electrocompetent Cells > 5 x 109 cfu/µg pUC DNA These cells are the most economical choice for standard cloning and library construction.
Chemically competent E. cloni 10G Chemically Competent Cells > 1 x 109 cfu/µg pUC DNA Highly efficient competent cells for routine cloning applications. Available in 96-well well format for higher throughput.
E. cloni 10G Chemically Competent Cells, Subcloning Grade > 1 x 106 cfu/µg pUC DNA The best value for simple cloning and plasmid propagation.
E. cloni 5-alpha Chemically Competent Cells > 1 x 108 cfu/µg pUC DNA Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.


E. cloni citations

Material available for download
E. cloni competent cells summary
E. cloni 10G and 10GF´ electrocompetent cells manual
E. cloni 10G chemically competent cells manual
E. cloni 5-alpha chemically competent cells manual
E. cloni 96-well chemically competent cells manual

DH5α and DH10B are trademarks of Life Technologies Inc

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
E. cloni® 10G SUPREME Electrocompetent Cells 60081-1 · Lucigen
60081-1
Lucigen
12 reactions (solos) £363.00
12 reactions (solos)
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E. cloni® 10G SUPREME Electrocompetent Cells 60081-2 · Lucigen
60081-2
Lucigen
24 reactions (solos) £641.00
24 reactions (solos)
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E. cloni® 10G SUPREME Electrocompetent Cells 60080-1 · Lucigen
60080-1
Lucigen
12 reactions (duos) £318.00
12 reactions (duos)
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E. cloni® 10G SUPREME Electrocompetent Cells 60080-2 · Lucigen
60080-2
Lucigen
24 reactions (duos) £584.00
24 reactions (duos)
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