- In this section:
- E. cloni competent cells summary
- E. cloni citations
The E. cloni® competent cells from Lucigen incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability. Available in chemically and electrocompetent formats in a range of high transformation efficiencies, the E.cloni cells are ideal as direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10 and XL1-Blue).
Benefits of the E. cloni range
• High yield optimised
• Directly replace standard cloning strains
• phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable
• Range of high electro competent transformation efficiencies (5 x 109 to 4 x 1010 cfu/µg)
• Range of high chemically competent transformation efficiencies (1 x 106 to 1 x 109 cfu/µg)
| Format | Name | Efficiency | Applications |
|---|---|---|---|
| Electrocompetent | E. cloni 10G SUPREME Electrocompetent Cells | > 4 x 1010 cfu/µg pUC DNA | SUPREME Cells are suitable for construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible. |
| E. cloni 10G & 10GF´ ELITE Electrocompetent Cells | > 2 x 1010 cfu/µg pUC DNA | ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA. | |
| E. cloni 10G CLASSIC Electrocompetent Cells | > 5 x 109 cfu/µg pUC DNA | These cells are the most economical choice for standard cloning and library construction. | |
| Chemically competent | E. cloni 10G Chemically Competent Cells | > 1 x 109 cfu/µg pUC DNA | Highly efficient competent cells for routine cloning applications. Available in 96-well well format for higher throughput. |
| E. cloni 10G Chemically Competent Cells, Subcloning Grade | > 1 x 106 cfu/µg pUC DNA | The best value for simple cloning and plasmid propagation. | |
| E. cloni 5-alpha Chemically Competent Cells | > 1 x 108 cfu/µg pUC DNA | Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening. |
Material available for download
E. cloni competent cells summary
E. cloni 10G and 10GF´ electrocompetent cells manual
E. cloni 10G chemically competent cells manual
E. cloni 5-alpha chemically competent cells manual
E. cloni 96-well chemically competent cells manual
DH5α and DH10B are trademarks of Life Technologies Inc
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| E. cloni® 10G SUPREME Electrocompetent Cells 60081-1 · Lucigen | 60081-1 Lucigen |
12 reactions (solos) |
£363.00
12 reactions (solos)
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| E. cloni® 10G SUPREME Electrocompetent Cells 60081-2 · Lucigen | 60081-2 Lucigen |
24 reactions (solos) |
£641.00
24 reactions (solos)
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| E. cloni® 10G SUPREME Electrocompetent Cells 60080-1 · Lucigen | 60080-1 Lucigen |
12 reactions (duos) |
£318.00
12 reactions (duos)
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| E. cloni® 10G SUPREME Electrocompetent Cells 60080-2 · Lucigen | 60080-2 Lucigen |
24 reactions (duos) |
£584.00
24 reactions (duos)
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