Routine cloning and library construction competent cells

The E. cloni® competent cells from Lucigen incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability. Available in chemically and electrocompetent formats in a range of high transformation efficiencies, the E.cloni cells are ideal as direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10 and XL1-Blue).

Benefits of the E. cloni range
• High yield optimised
• Directly replace standard cloning strains
• phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable
• Range of high electro competent transformation efficiencies (5 x 10⁹ to 4 x 10¹⁰ cfu/µg)
• Range of high chemically competent transformation efficiencies (1 x 10⁶ to 1 x 10⁹ cfu/µg)

Format	Name	Efficiency	Applications
Electrocompetent	E. cloni 10G SUPREME Electrocompetent Cells	> 4 x 10¹⁰ cfu/µg pUC DNA	SUPREME Cells are suitable for construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.
	E. cloni 10G & 10GF´ ELITE Electrocompetent Cells	> 2 x 10¹⁰ cfu/µg pUC DNA	ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA.
	E. cloni 10G CLASSIC Electrocompetent Cells	> 5 x 10⁹ cfu/µg pUC DNA	These cells are the most economical choice for standard cloning and library construction.
Chemically competent	E. cloni 10G Chemically Competent Cells	> 1 x 10⁹ cfu/µg pUC DNA	Highly efficient competent cells for routine cloning applications. Available in 96-well well format for higher throughput.
	E. cloni 10G Chemically Competent Cells, Subcloning Grade	> 1 x 10⁶ cfu/µg pUC DNA	The best value for simple cloning and plasmid propagation.
	E. cloni 5-alpha Chemically Competent Cells	> 1 x 10⁸ cfu/µg pUC DNA	Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.

E. cloni citations

Material available for download
E. cloni competent cells summary
E. cloni 10G and 10GF´ electrocompetent cells manual
E. cloni 10G chemically competent cells manual
E. cloni 5-alpha chemically competent cells manual
E. cloni 96-well chemically competent cells manual

DH5α and DH10B are trademarks of Life Technologies Inc

Products

Note: product availability depends on country - see product detail page.

Details	Cat number & supplier	Size	Price
E. cloni 10G Supreme Electrocompetent Cells 60081-1 · Lucigen	60081-1 Lucigen	12 reactions solos	£454.00 12 reactions solos view
E. cloni 10G Supreme Electrocompetent Cells 60080-1 · Lucigen	60080-1 Lucigen	12 reactions duos	£398.00 12 reactions duos view
E. cloni 10G Supreme Electrocompetent Cells 60080-2 · Lucigen	60080-2 Lucigen	24 reactions duos	£732.00 24 reactions duos view
E. cloni 10GF' Electrocompetent Cells 60061-1 · Lucigen	60061-1 Lucigen	12 reactions duos	£258.00 12 reactions duos view
E. cloni 10GF' Electrocompetent Cells 60061-2 · Lucigen	60061-2 Lucigen	24 reactions duos	£445.00 24 reactions duos view
E. cloni 10G Elite Electrocompetent Cells 60052-1 · Lucigen	60052-1 Lucigen	12 reactions duos	£256.00 12 reactions duos view
E. cloni 10G Elite Electrocompetent Cells 60052-2 · Lucigen	60052-2 Lucigen	24 reactions duos	£440.00 24 reactions duos view
E. cloni 10G Elite Electrocompetent Cells 60052-3 · Lucigen	60052-3 Lucigen	24 reactions sixpacks	£339.00 24 reactions sixpacks view
E. cloni 10G Elite Electrocompetent Cells 60052-4 · Lucigen	60052-4 Lucigen	48 reactions sixpacks	£562.00 48 reactions sixpacks view
E. cloni 10G Classic Electrocompetent Cells 60117-1 · Lucigen	60117-1 Lucigen	24 reactions sixpacks	£301.00 24 reactions sixpacks view
E. cloni 10G Classic Electrocompetent Cells 60117-2 · Lucigen	60117-2 Lucigen	48 reactions sixpacks	£537.00 48 reactions sixpacks view
E. cloni 10G Chemically Competent Cells 60107-1 · Lucigen	60107-1 Lucigen	12 reactions duos	£190.00 12 reactions duos view
E. cloni 10G Chemically Competent Cells 60107-2 · Lucigen	60107-2 Lucigen	24 reactions duos	£332.00 24 reactions duos view
E. cloni 10G Chemically Competent Cells Subcloning Grade 60108-1 · Lucigen	60108-1 Lucigen	48 reactions	£109.00 48 reactions view
E. cloni 5-alpha Chemically Competent Cells 60602-1 · Lucigen	60602-1 Lucigen	12 reactions duos	£170.00 12 reactions duos view

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Routine cloning and library construction competent cells

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