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RNA from blood: Quick-RNA

The Quick-RNA™ Whole Blood kit from Zymo Research utilises DNA/RNA Shield™ to enable the rapid isolation of total RNA from whole or partitioned blood, or a cell pellet after red blood cell lysis. Using Zymo-Spin column technology, samples are pre-filtered on the Zymo-Spin IIICG Column, then purified and concentrated on the Zymo-Spin IC Column. Purified RNA can be eluted into as little as 6 µl of RNase-free water and is ready for any downstream applications including RT-PCR and sequencing.

Samples can include up to 1 ml mammalian whole-blood, plasma, or serum. The kit is also compatible with pelleted blood cells (PBMCs, WBCs, buffy coat, pelleted sample from PAXgene™ Blood RNA Tube, etc.) and nucleated blood.

Benefits of using the Quick-RNA Whole Blood kit
• Total RNA (including small/micro RNA ≥ 17nt) recovered with no sample loss
• Up to 10 µg RNA purified
• DNAse I included
• Elute in as little as 6 µl
• Compatible with mammalian whole-blood, plasma and serum as well as pelleted blood cells including PBMCs, WBCs and buffy coat samples

Quick-RNA Whole Blood kit gives high quality RNA

High-quality  RNA  was  extracted  from  human  whole  blood  using  the Quick-RNA™  Whole  Blood  Kit.  Blood  was  stored  in  DNA/RNA  Shield™  at  ambient  temperatures  for  two  days  prior  to  extraction  (n=4).  RNA  was  visualized  using  the  Agilent    2200  Tapestation®system.

High-quality  RNA  was  extracted  from  human  whole  blood  using  the Quick-RNA  Whole  Blood  Kit.  Blood  was  stored  in  DNA/RNA  Shield  at  ambient  temperatures  for  two  days  prior  to  extraction  (n=4).  RNA  was  visualised  using  the  Agilent    2200  Tapestation® system.

Quick-RNA Whole Blood kit gives superior RNA yields

Amount  of  RNA  extracted  from  1  ml  of  human  whole  blood  was  significantly  higher  using  the Quick-RNA™  Whole  Blood  Kit  vs  the  Supplier Q kit (n=3).

Amount  of  RNA  extracted  from  1  ml  of  human  whole  blood  was  significantly  higher  using  the Quick-RNA  Whole  Blood  Kit  vs  the  Supplier Q kit (n=3).

DNA/RNA  Shield stabilises blood samples

RT-qPCR shows the Zymo Research workflow stabilizes RNA, while the Supplier Q workflow leads to degradation. Whole blood    was  stored  up  to  7  days  at  ambient  temperatures  and extracted at the indicated time points using the Zymo Research or Supplier Q preservatives and workflows

High-quality  RNA  was  extracted  from  human  whole  blood  using  the Quick-RNA  Whole  Blood  Kit.  Blood  was  stored  in  DNA/RNA  Shield  at  ambient  temperatures  for  two  days  prior  to  extraction  (n=4).  RNA  was  visualized  using  the  Agilent    2200  Tapestation® system.

Material available for download
Quick-RNA Whole Blood protocol
Quick-RNA Whole Blood kit datasheet

Quick-RNA Whole Blood citations

Paxgene is a trademark of Preanalytix GmbH. Tapestation is a trademark of Agilent Technologies.

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Quick-RNA Whole Blood R1201 · Zymo Research
R1201
Zymo Research
50 preparations £357.00
50 preparations
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Quick-DNA/RNA Blood Tube Kit R1151 · Zymo Research
R1151
Zymo Research
50 preparations £460.00
50 preparations
view
DNA/RNA Shield Blood Collection Tube R1150 · Zymo Research
R1150
Zymo Research
50 pack £549.00
50 pack
view