The Quick-RNA™ Whole Blood kit from Zymo Research utilises DNA/RNA Shield™ to enable the rapid isolation of total RNA from whole or partitioned blood, or a cell pellet after red blood cell lysis. Using Zymo-Spin column technology, samples are pre-filtered on the Zymo-Spin IIICG Column, then purified and concentrated on the Zymo-Spin IC Column. Purified RNA can be eluted into as little as 6 µl of RNase-free water and is ready for any downstream applications including RT-PCR and sequencing.
Samples can include up to 1 ml mammalian whole-blood, plasma, or serum. The kit is also compatible with pelleted blood cells (PBMCs, WBCs, buffy coat, pelleted sample from PAXgene™ Blood RNA Tube, etc.) and nucleated blood.
Benefits of using the Quick-RNA Whole Blood kit
• Total RNA (including small/micro RNA ≥ 17nt) recovered with no sample loss
• Up to 10 µg RNA purified
• DNAse I included
• Elute in as little as 6 µl
• Compatible with mammalian whole-blood, plasma and serum as well as pelleted blood cells including PBMCs, WBCs and buffy coat samples
Quick-RNA Whole Blood kit gives high quality RNA
High-quality RNA was extracted from human whole blood using the Quick-RNA Whole Blood Kit. Blood was stored in DNA/RNA Shield at ambient temperatures for two days prior to extraction (n=4). RNA was visualised using the Agilent 2200 Tapestation® system.
Quick-RNA Whole Blood kit gives superior RNA yields
Amount of RNA extracted from 1 ml of human whole blood was significantly higher using the Quick-RNA Whole Blood Kit vs the Supplier Q kit (n=3).
DNA/RNA Shield stabilises blood samples
High-quality RNA was extracted from human whole blood using the Quick-RNA Whole Blood Kit. Blood was stored in DNA/RNA Shield at ambient temperatures for two days prior to extraction (n=4). RNA was visualized using the Agilent 2200 Tapestation® system.
Paxgene is a trademark of Preanalytix GmbH. Tapestation is a trademark of Agilent Technologies.
Note: product availability depends on country - see product detail page.
|Details||Cat number & supplier||Size||Price|
|Quick-RNA Whole Blood R1201 · Zymo Research||
|Quick-DNA/RNA Blood Tube Kit R1151 · Zymo Research||
|DNA/RNA Shield Blood Collection Tube R1150 · Zymo Research||