- In this section:
- riboPOOL probes protocol
- riboPOOL kit protocol
- riboPOOL citations
riboPOOLs are an affordable and flexible solution for ribosomal RNA (rRNA) depletion or other custom RNAs from any species from siTOOLs. Composed of high complexity pools of optimally-designed biotinylated DNA probes, riboPOOLs allow the efficient removal of abundant rRNAs from total RNA samples isolated from prokaryotic or eukaryotic cells or tissues.
Benefits of using riboPOOL
• Highly efficient and specific
• Any species
• Low cost solution
• Deplete custom RNA
• Applicable for nonpolyadenylated RNA
• Custom scales available
riboPOOLs can be provided as either:
• Probes alone with nuclease-free water. Please refer to the protocol for reagents required.
• Complete riboPOOL kits that include buffer, steptavidin-magnetic beads & ethanol precipitation reagents.
riboPOOL workflow
riboPOOL technology
riboPOOLs consist of complex mixtures of single-stranded 3’biotinylated oligonucleotide-probes designed to specifically hybridise with all ribosomal RNAs of the target species. Ribosomal RNAs bound to riboPOOL-probes are efficiently removed from the samples by streptavidin-conjugated magnetic beads. riboPOOLs work independent of RNA polyadenylation and can be applied for any downstream application e.g. NGS library preparation.
riboPOOL formats
Choose from a diverse list of ready-made riboPOOLs or custom-design a riboPOOL for any species or RNA of choice.
riboPOOLs may be combined in custom ratios to form a combination riboPOOL that can be used for samples across multiple species
Material available for download
riboPOOL kit protocol
riboPOOL probes protocol
riboPOOL brochure
Pan-prokaryote riboPOOL flyer
Pan-Plant flyer
New pan-prokaryote riboPOOLs flyer
riboPOOL application note - efficient ribosomal RNA depletion across multiple species & input amounts in 70 mins
Ribo-Zero is a trademark of Illumina, Inc