Quick-16S Full-Length Library Prep Kit

Accelerate your sequencing workflow with the Quick-16S Full-Length Library Prep Kit, designed for PacBio and Oxford Nanopore platforms. With just 30 minutes of hands-on time, you can generate pooled, purified long-read libraries ready for high-quality sequencing.

This kit features 96-well premixed reagent plates, available in four distinct barcode formats, enabling seamless multiplexing of up to 384 samples. Its optimised one-step PCR workflow eliminates complexity - simply add DNA and amplify. The pre-formulated PCR premix supports qPCR compatibility, includes dead volume for automated liquid handling, and leverages highly stable reagents that allow for room-temperature PCR setup without requiring ice or cold blocks.

Benefits of the Quick-16S full-length library prep kit?

• Ultra-Fast Workflow – go from DNA to pooled libraries in just 30 minutes of hands-on time.
• Effortless PCR Setup – preloaded, premixed amplification plates ensure a one-step reaction - just add DNA.
• Broad Compatibility – works seamlessly with PacBio and Oxford Nanopore Technologies for maximum flexibility.

Applications of the Quick 16S full-length library prep kit

• Microbiome profiling – enables high-resolution analysis of microbial communities using full-length 16S rRNA sequencing.
• Bacterial & archaeal taxonomy – provides accurate species- and strain-level identification in complex samples.
• Environmental microbiology – ideal for studying microbial diversity in soil, water, and other environmental samples.
• Clinical & human microbiome research – supports investigations into the role of microbiota in human health and disease.
• Agricultural & veterinary microbiology – facilitates research on microbial interactions in livestock, crops, and food safety.
• Infectious disease research – helps characterise pathogenic and commensal bacteria in medical and epidemiological studies.
• Industrial & biotechnological applications – useful for monitoring microbial communities in fermentation, bioremediation, and other bioprocesses.

Accurate microbial profiling

Each Quick-16S Full-Length Library Prep Kit includes our ZymoBIOMICS® Microbial Community DNA Standard, which contains the extracted, quantified, and homogenized DNA of 8 organisms serving as a mock community.

Figure 2. Accurate profiling of ZymoBIOMICS® Microbial Community DNA Standard. Full-length 16S rRNA  gene sequencing profiles accurately reflect the theoretical composition of the ZymoBIOMICS® Microbial Community DNA Standard, demonstrating little amplification bias based on microbial genomic G/C content.

Materials for download

Quick-16S Full-Length Library Prep Kit - Protocol

Quick-16S Full-Length Library Prep Kit - Data Sheet

Appendix A - Guide for preparing more than 96 samples