High-throughput 3' mRNA library preparation: QuantSeq POOL

QuantSeq POOL combines Lexogen’s QuantSeq 3’ mRNA technology with early sample pooling and barcoding, offering a solution for large-scale gene expression screening projects. If the largest available set of 384 unique dual indexes (UDIs) is combined with all 96 i1 barcodes, the workflow can produce 36,864 individually barcoded samples, allowing for high levels of multiplexing and sequencing throughput.

During the first library generation step, sample barcodes are introduced to enable subsequent pooling. Unique Molecular Identifiers (UMIs) are also incorporated at this step to allow for elimination of PCR duplicates. During the library amplification step, additional Unique Dual Indices (UDIs) can be introduced at the i5 and i7 positions of QuantSeq-Pool libraries for higher multiplexing capacity.

Benefits of QuantSeq POOL

• Fast: Accelerate your workflow with early pooling and batch processing of up to 96 samples in one reaction.
• Scalable throughput: Process large-scale projects with multiplexing of up to 36,864 samples, supported by an automation-friendly protocol.
• Cost efficient: Reduce sequencing and consumable costs up to tenfold.
• Reproducible: Reduce technical variability and consistently process large numbers of samples in parallel.
• Accurate: Eliminate PCR duplicates with built-in UMIs for high-precision expression profiling.
• Simple data analysis: Save time and computing capacity by simply counting mapped reads to calculate gene expression.

Robust gene detection at low sequencing depths

QuantSeq POOL detects highly expressed genes even at low sequencing depths and does so in a consistent and reproducible manner.

Materials for download

QuantSeq POOL product flyer

QuantSeq POOL user guide