3’ mRNA-Seq library preparation: QuantSeq

QuantSeq 3’ mRNA-Seq Library Prep kits from Lexogen offers cost-efficient analysis of differential gene expression via 3’ mRNA-seq library preparation. Requiring fewer reads per samples and capable of multiplexing a high number of samples on a single sequencing lane, the 3’ mRNA-Seq technology simplifies your gene expression projects with no need for additional pre-processing steps.

Lexogen offers a high-throughput version, with optional dual indexing (i5 and i7 indices) allowing for up to 9,216 samples to be multiplexed in a single lane.

Benefits of using the QuantSeq mRNA-seq library prep kits
• Suitable for analysis of low input (as low as 1ng) and low quality RNA samples, such as FFPE samples
• Significantly less computational resources required with large multiplexing capacity
• Simple workflow to generate ready-to sequence NGS libraries in only 4.5 hours, with <2 hours hands-on time
• No bias for transcript length
• BlueBee® data analysis included

QuantSeq 3’ mRNA-Seq mapping of transcript end sites

QuantSeq read coverage vs normalized transcript length of NGS libraries derived from FFPE-RNA (blue) and cryo-preserved RNA (red) samples. By using longer reads QuantSeq FWD allows to precisely pinpoint the 3’ end of poly(A) RNA and thus obtain accurate information about the 3’ UTR. 

Applications
• Genome-wide gene expression analysis studies
• RNA sequencing or transcriptomic analysis

System compatibility
• HiSeq® 2000/2500/3000/4000
• NextSeq® 500/550
• NovaSeq® 6000
• MiSeq®
• MiniSeq®
• Genome Analyzer®

Material available for download
QuantSeq application note
QuantSeq application note - RNA quantification
QuantSeq Illumina® user guide
QuantSeq product flyer

Illumina, HiSeq, NextSeq, NovaSeq, MiSeq, MiniSeq, Genome Analyzer and BlueBee are registered trademarks of Illumina, Inc