QuantSeq 3’ mRNA-Seq Library Prep kits from Lexogen offers cost-efficient analysis of differential gene expression via 3’ mRNA-seq library preparation. Requiring fewer reads per samples and capable of multiplexing a high number of samples on a single sequencing lane, the 3’ mRNA-Seq technology simplifies your gene expression projects with no need for additional pre-processing steps.
Lexogen offers a high-throughput version, with optional dual indexing (i5 and i7 indices) allowing for up to 9,216 samples to be multiplexed in a single lane.
Benefits of using the QuantSeq mRNA-seq library prep kits
• Suitable for analysis of low input (as low as 1ng) and low quality RNA samples, such as FFPE samples
• Significantly less computational resources required with large multiplexing capacity
• Simple workflow to generate ready-to sequence NGS libraries in only 4.5 hours, with <2 hours hands-on time
• No bias for transcript length
• BlueBee® data analysis included
QuantSeq 3’ mRNA-Seq mapping of transcript end sites
QuantSeq read coverage vs normalized transcript length of NGS libraries derived from FFPE-RNA (blue) and cryo-preserved RNA (red) samples. By using longer reads QuantSeq FWD allows to precisely pinpoint the 3’ end of poly(A) RNA and thus obtain accurate information about the 3’ UTR.
Applications
• Genome-wide gene expression analysis studies
• RNA sequencing or transcriptomic analysis
System compatibility
• HiSeq® 2000/2500/3000/4000
• NextSeq® 500/550
• NovaSeq® 6000
• MiSeq®
• MiniSeq®
• Genome Analyzer®
Material available for download
QuantSeq application note
QuantSeq application note - RNA quantification
QuantSeq Illumina® user guide
QuantSeq product flyer
Illumina, HiSeq, NextSeq, NovaSeq, MiSeq, MiniSeq, Genome Analyzer and BlueBee are registered trademarks of Illumina, Inc
Products
Note: product availability depends on country - see product detail page.
Details | Cat number & supplier | Size | Price |
QuantSeq 3‘ mRNA-Seq Library Prep Kit (FWD) HT with i5 Dual Indexing Add-on Kit (5001-5004) 015.384 · Lexogen | 015.384 Lexogen |
384 preparations |
£8014.00
384 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Sets A1-A4 (UDI12A_0001-0384) 115.384 · Lexogen | 115.384 Lexogen |
384 preparations |
£8802.00
384 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set B1 114.96 · Lexogen | 114.96 Lexogen |
96 preparations |
£2346.00
96 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set A4 (UDI12A_0289-0384) 131.96 · Lexogen | 131.96 Lexogen |
96 preparations |
£2346.00
96 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set A3 (UDI12A_0193-0288) 130.96 · Lexogen | 130.96 Lexogen |
96 preparations |
£2346.00
96 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set A2 (UDI12A_0097-0192) 129.96 · Lexogen | 129.96 Lexogen |
96 preparations |
£2346.00
96 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set A1 (UDI12A_0001-0096) 113.96 · Lexogen | 113.96 Lexogen |
96 preparations |
£2346.00
96 preparations
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QuantSeq 3‘ mRNA-Seq Library Prep Kit (REV) with Custom Sequencing Primer 016.96 · Lexogen | 016.96 Lexogen |
96 preparations |
£2296.00
96 preparations
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QuantSeq 3‘ mRNA-Seq Library Prep Kit (REV) with Custom Sequencing Primer 016.24 · Lexogen | 016.24 Lexogen |
24 preparations |
£691.00
24 preparations
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QuantSeq 3‘ mRNA-Seq Library Prep Kit (FWD) 015.96 · Lexogen | 015.96 Lexogen |
96 preparations |
£2196.00
96 preparations
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QuantSeq 3‘ mRNA-Seq Library Prep Kit (FWD) 015.2x96 · Lexogen | 015.2x96 Lexogen |
2 x 96 preparations |
£4007.00
2 x 96 preparations
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QuantSeq 3‘ mRNA-Seq Library Prep Kit (FWD) 015.24 · Lexogen | 015.24 Lexogen |
24 preparations |
£698.00
24 preparations
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QuantSeq-Pool Sample-Barcoded 3’ mRNA-Seq Library Prep Kit for Illumina 139.96 · Lexogen | 139.96 Lexogen |
96 preparations |
£2207.00
96 preparations
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