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3’ mRNA-Seq library preparation: QuantSeq

QuantSeq 3’ mRNA-Seq Library Prep kits from Lexogen offers cost-efficient analysis of differential gene expression via 3’ mRNA-seq library preparation. Requiring fewer reads per samples and capable of multiplexing a high number of samples on a single sequencing lane, the 3’ mRNA-Seq technology simplifies your gene expression projects with no need for additional pre-processing steps.

Lexogen offers a high-throughput version, with optional dual indexing (i5 and i7 indices) allowing for up to 9,216 samples to be multiplexed in a single lane.

Benefits of using the QuantSeq mRNA-seq library prep kits
• Suitable for analysis of low input (as low as 1ng) and low quality RNA samples, such as FFPE samples
Significantly less computational resources required with large multiplexing capacity
 Simple workflow to generate ready-to sequence NGS libraries in only 4.5 hours, with <2 hours hands-on time
• No bias for transcript length
• BlueBee® data analysis included

QuantSeq 3’ mRNA-Seq mapping of transcript end sites

Lexogen coverage read

QuantSeq read coverage vs normalized transcript length of NGS libraries derived from FFPE-RNA (blue) and cryo-preserved RNA (red) samples. By using longer reads QuantSeq FWD allows to precisely pinpoint the 3’ end of poly(A) RNA and thus obtain accurate information about the 3’ UTR. 

Applications
• Genome-wide gene expression analysis studies
• RNA sequencing or transcriptomic analysis

System compatibility
• HiSeq® 2000/2500/3000/4000
• NextSeq® 500/550
• NovaSeq® 6000
• MiSeq®
• MiniSeq®
• Genome Analyzer®

Material available for download
QuantSeq application note
QuantSeq application note - RNA quantification
QuantSeq Illumina® user guide
QuantSeq product flyer

Illumina, HiSeq, NextSeq, NovaSeq, MiSeq, MiniSeq, Genome Analyzer and BlueBee are registered trademarks of Illumina, Inc

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
QuantSeq 3‘ mRNA-Seq Library Prep Kit (FWD) HT with i5 Dual Indexing Add-on Kit (5001-5004) 015.384 · Lexogen
015.384
Lexogen
384 preparations £8014.00
384 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Sets A1-A4 (UDI12A_0001-0384) 115.384 · Lexogen
115.384
Lexogen
384 preparations £8802.00
384 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set B1 114.96 · Lexogen
114.96
Lexogen
96 preparations £2346.00
96 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set A4 (UDI12A_0289-0384) 131.96 · Lexogen
131.96
Lexogen
96 preparations £2346.00
96 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set A3 (UDI12A_0193-0288) 130.96 · Lexogen
130.96
Lexogen
96 preparations £2346.00
96 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set A2 (UDI12A_0097-0192) 129.96 · Lexogen
129.96
Lexogen
96 preparations £2346.00
96 preparations
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QuantSeq 3’ mRNA-Seq Library Prep Kit FWD with UDI 12 nt Set A1 (UDI12A_0001-0096) 113.96 · Lexogen
113.96
Lexogen
96 preparations £2346.00
96 preparations
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QuantSeq 3‘ mRNA-Seq Library Prep Kit (REV) with Custom Sequencing Primer 016.96 · Lexogen
016.96
Lexogen
96 preparations £2296.00
96 preparations
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QuantSeq 3‘ mRNA-Seq Library Prep Kit (REV) with Custom Sequencing Primer 016.24 · Lexogen
016.24
Lexogen
24 preparations £691.00
24 preparations
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QuantSeq 3‘ mRNA-Seq Library Prep Kit (FWD) 015.96 · Lexogen
015.96
Lexogen
96 preparations £2196.00
96 preparations
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QuantSeq 3‘ mRNA-Seq Library Prep Kit (FWD) 015.2x96 · Lexogen
015.2x96
Lexogen
2 x 96 preparations £4007.00
2 x 96 preparations
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QuantSeq 3‘ mRNA-Seq Library Prep Kit (FWD) 015.24 · Lexogen
015.24
Lexogen
24 preparations £698.00
24 preparations
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QuantSeq-Pool Sample-Barcoded 3’ mRNA-Seq Library Prep Kit for Illumina 139.96 · Lexogen
139.96
Lexogen
96 preparations £2207.00
96 preparations
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