The QuantSeq 3’ mRNA-Seq Library Prep kit V2 from Lexogen offers cost-efficient analysis of differential gene expression via 3’ mRNA-seq library preparation. Requiring fewer reads per sample and capable of multiplexing a high number of samples on a single sequencing lane, the 3’ mRNA-Seq technology simplifies your gene expression projects with no need for additional pre-processing steps.
The kit features Lexogen's patented 12-nt long Unique Dual Indices (UDIs), providing increased robustness and multiplexing capabilities, resulting in increased read capacity for next-generation sequencing platforms. UDI add-ons can also be purchased separately to complement other QuantSeq kits.
Benefits of using the QuantSeq V2 mRNA-seq library prep kits
- Suitable for analysis of low input (as low as 1ng) and low-quality RNA samples, such as FFPE samples
- Significantly less computational resources required with large multiplexing capacity
- Simple workflow to generate ready-to-sequence NGS libraries in only 4.5 hours, with <2 hours hands-on time
- No bias for transcript length
- Includes high quality in-house data analytics with an activation code for free data analysis runs
QuantSeq has been cited in over 1600 publications since its launch, quickly becoming a trusted and reliable technology for the scientific community.
QuantSeq 3’ mRNA-Seq V2 mapping of transcript end sites
QuantSeq V2 read coverage vs normalized transcript length of NGS libraries derived from FFPE-RNA (blue) and cryo-preserved RNA (red) samples. By using longer reads QuantSeq FWD allows to precisely pinpoint the 3’ end of poly(A) RNA and thus obtain accurate information about the 3’ UTR.
Applications of Lexogen's QuantSeq
- Genome-wide gene expression analysis studies
- RNA sequencing or transcriptomic analysis
System compatibility
- HiSeq® 2000/2500/3000/4000
- NextSeq® 500/550
- NovaSeq® 6000
- MiSeq®
- MiniSeq®
- Genome Analyzer®
Material available for download
QuantSeq V2 application note
QuantSeq V2 application note - RNA quantification
QuantSeq V2 Illumina® user guide
QuantSeq V2 product flyer
Illumina, HiSeq, NextSeq, NovaSeq, MiSeq, MiniSeq and Genome Analyzer are registered trademarks of Illumina, Inc
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