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Phage display competent cells

The electrocompetent cells for phage display from Lucigen were developed using a proprietary protocol that allows greater E. coli host strain transformation efficiencies. These competent cells outperform competitor cells by up to 5-fold, with the highest transformation efficiencies available on the market.

TG1 electrocompetent cells
An amber suppressor strain (supE) prepared as highly efficient (≥4×1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression.

SS320 electrocompetent cells
A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4×1010 cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.

ER2738 electrocompetent cells
An amber suppressor strain (glnV) prepared as highly efficient (≥2×1010 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New England Biolab's Ph.D.™ Phage display kits.

MC1061 F- electrocompetent cells
A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for reinfection by filamentous phage. 

Benefits of using these competent cells
• Highest efficiency TG1 competent cells available (≥4 × 1010 cfu/µg)
• Select from amber suppressor and non-amber suppressor strains
• Generate larger libraries with greater diversity to speed discovery
• Bulk custom dispensing available 

Transformation efficiency of Lucigen's electrocompetent bacterial cells for phage display compared to competitor's specification

Transformation efficiency of Lucigen's electrocompetent bacterial cells for phage display compared to competitor's specification

 Competent cells for phage display libraries

 
Cell lines Transformation efficiency (cfu/µg pUC DNA) Cloning methylated DNA Blue/white screening* Amber suppressor strain F' plasmid
TG1 ≥4 x 1010
SS320 (MC106 F') ≥4 x 1010  
ER2738 ≥2 x 1010
MC1061 F- ≥4 x 1010    

*IPTG induction required

Free samples
Determine which strain* performs best for your library construction. Request a free phage display ElectroCombo pack.

Material available to download
Phage Display Electrocompetent Cells (TG1, SS320 & ER2738) manual
MC1061 F- Electrocompetent Cells manual
Competent Cells for larger phage display libraries
Application note: High efficiency E. coli strains for phage display combinatorial peptide libraries
Maximise your success with Lucigen competent cells

*The MC1061 F- strain is not included in the phage display ElectroCombo sample pack 

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
TG1 Electrocompetent Cells 60502-1 · Lucigen
60502-1
Lucigen
12 reactions (duos) £242.00
12 reactions (duos)
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TG1 Electrocompetent Cells 60502-2 · Lucigen
60502-2
Lucigen
24 reactions (duos) £419.00
24 reactions (duos)
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SS320 (MC1061 F′) Electrocompetent Cells 60512-1 · Lucigen
60512-1
Lucigen
12 reactions (duos) £254.00
12 reactions (duos)
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SS320 (MC1061 F′) Electrocompetent Cells 60512-2 · Lucigen
60512-2
Lucigen
24 reactions (duos) £421.00
24 reactions (duos)
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ER2738 Electrocompetent Cells 60522-1 · Lucigen
60522-1
Lucigen
12 reactions (duos) £228.00
12 reactions (duos)
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ER2738 Electrocompetent Cells 60522-2 · Lucigen
60522-2
Lucigen
24 reactions (duos) £397.00
24 reactions (duos)
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MC1061 F- Electrocompetent Cells 60514-1 · Lucigen
60514-1
Lucigen
12 reactions (duos) £222.00
12 reactions (duos)
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MC1061 F- Electrocompetent Cells 60514-2 · Lucigen
60514-2
Lucigen
24 reactions (duos) £384.00
24 reactions (duos)
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