PathPlex IHC multiplex antibody panels

The PathPlex™ IHC multiplex antibody panels from Bethyl allow for the visualisation of multiple targets within a single tissue section. Unlike traditional IHC methods, which cannot differentiate between multiple cells types or targets without serial sections, these panels quickly detect multiple antigens in a single section to gain a clear understanding of the tumour microenvironment whilst preserving tissue architecture.

Benefits of using IHC PathPlex panels
• Quickly detect multiple antigens in a single tissue section
• Tissue architecture is preserved
• Ideal for IHC in limited samples
• Obtain co-expression & spatial data from within the tumour microenvironment
• Low-level binding sites detected
• Intensity for each target measured – signals can be overlaid & seen as single or multi- channel
• Compatible with any mouse & rabbit primary antibody combination

Pathplex Lymphocyte Panel

Detection of human CD3 (yellow), CD8 (red), and CD20 (green) using PathPlex Lymphocyte panel in FFPE tonsil by IHC-IF. Rabbit anti-CD3e recombinant monoclonal [BL-298-5D12] (A700-016), rabbit anti-CD8a recombinant monoclonal [BLR044F] (A700-044), mouse anti-CD20 monoclonal [L26] (A500-017A). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P) and HRP-conjugated goat anti-mouse IgG (A90-116P). Substrate: Opal™ 520, 620, and 690. Counterstain: DAPI (blue)

PathPlex T, B & M Panel

Detection of human CD3 (green), CD20 (magenta), CD68 (yellow) using PathPlex T, B & M Panel, and Cytokeratin (red) in FFPE tonsil by IHC-IF. Antibodies: CD3 (A700-016), CD20 (A500-017A), CD68 (A500-018A), Cytokeratin (A500-019A). Substrate: Opal™ reagent. Counterstain: DAPI (blue).

Formats available

Panel	Targets	Application
T-cell immuno-oncology panel	CD3E, CD8 alpha, PD-L1	Base panel for checkpoint inhibition and T-cell activation. This panel measures number of total T-cells (CD3) and activated T-cells (CD8) in context of one of the major checkpoint inhibitors, PD-L1. Can be combined with other markers such as cytokeratin to also label tumour cells or CD68 to measure macrophages.
Lymphocyte panel	CD3E, CD8 alpha, CD20	Base panel for lymphocyte infiltration. This panel measures number of total T-cells (CD3), activated T-cells (CD8), total B-cells (CD20). Can be combined with other markers such as cytokeratin to also label tumour cells.
T, B & M panel	CD3E, CD68, CD20	Base panel for measuring lymphocyte and macrophage cells. This panel detects T-cells (CD3), B-cells (CD20) and macrophages (CD68). Can be combined with other markers such as cytokeratin to also label tumour cells.

Applications
• Immunohistochemistry Immunofluorescence (IHC-IF)
• Multiplex Immunofluorescence (mIF)

Material available for download
PathPlex IHC Multiplex Antibody Panels brochure

Products

Note: product availability depends on country - see product detail page.

Details	Cat number & supplier	Size	Price
PathPlex® Panel 1 (CD3E, CD8 alpha, PD-L1) A810-001 · Bethyl Laboratories, Inc.	A810-001 Bethyl Laboratories, Inc.	1 panel	£1015.00 1 panel view
PathPlex® Panel 2 (CD3E, CD8 alpha, CD20) A810-002 · Bethyl Laboratories, Inc.	A810-002 Bethyl Laboratories, Inc.	1 panel	£1015.00 1 panel view
PathPlex® Panel 3 (CD3E, CD68, CD20) A810-003 · Bethyl Laboratories, Inc.	A810-003 Bethyl Laboratories, Inc.	1 panel	£1015.00 1 panel view

In this section

PathPlex IHC Multiplex Antibody Panels brochure