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NxGen Grade T4 DNA Ligases

The NxGen® T4 DNA Ligases from LGC Biosearch (Lucigen) come in a range of highly pure grades specifically for next-generation sequencing (NGS). This enzyme catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA and efficiently joins blunt and cohesive ends, and repairs single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids. 

T4 DNA ligase version Sizes Ligase concentration Buffer provided
Low concentration ligase 1,500 U
7,500 U
2 U/µL 10X T4 DNA Ligase Buffer
High Concentration Rapid Kit 1,500 U
7,500 U
10 U/µL 2X Rapid Ligation Buffer
10X T4 DNA Ligase Buffer


The NxGen Grade T4 DNA Ligase is ≥ 95% pure

The NxGen Grade T4 DNA Ligase is ≥ 95% pure

The purity of the T4 DNA ligase is ≥95% as judged by SDS-polyacrylamide gel electrophoresis with Coomassie blue staining.  Molecular weight markers are in Lane 1.  T4 DNA ligase was loaded at 5 units (Lane 2) and 10 units (Lane 3).

The T4 DNA ligase is free of detectable of exo- and endonuclease activities

The T4 DNA ligase is free of detectable of exo- and endonuclease activities

The T4 DNA ligase is free of detectable of exo- and endonuclease activities, as judged by agarose gel electrophoresis following incubation of 10 units of enzyme for 16 hours at 37 °C with 1 µg of HindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA (Lanes 5 and 6).  Molecular weight markers are in Lanes 1 and 2.

Material Available for Download
NxGen T4 DNA Ligase High Concentration Rapid Kit Manual
NxGen T4 DNA Ligase (Low Concentration) Manual

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
NxGen® T4 DNA Ligase (Low Concentration) 30241-1 · Lucigen
30241-1
Lucigen
1500 units £155.00
1500 units
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NxGen® T4 DNA Ligase (Low Concentration) 30241-2 · Lucigen
30241-2
Lucigen
7500 units £633.00
7500 units
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NxGen® T4 DNA Ligase (High Concentration) 30243-1 · Lucigen
30243-1
Lucigen
1500 units £237.00
1500 units
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NxGen® T4 DNA Ligase (High Concentration) 30243-2 · Lucigen
30243-2
Lucigen
7500 units £946.00
7500 units
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