The NxGen® M-MuLV Reverse Transcriptase from LGC Biosearch (Lucigen) is a highly-pure, RNA-dependent DNA polymerase which shows no measurable 3′ to 5′ proofreading function. The enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.
Features of the NxGen M-MuLV Reverse Transcriptase
|Unit Concentration||200,000 U/mL|
|SS Exonuclease||2,000 U <5.0% released|
|DS Exonuclease||2,000 U <0.5% released|
|Endonuclease||2,000 U <10% converted|
|E. coli 16S rDNA Contamination||2000 U <10 copies|
Unit Definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
Material available for download
NxGen M-MuLV Reverse Transcriptase manual
Note: product availability depends on country - see product detail page.