NxGen Grade M-MuLV Reverse Transcriptase

The NxGen® M-MuLV Reverse Transcriptase from LGC Biosearch (Lucigen) is a highly-pure, RNA-dependent DNA polymerase which shows no measurable 3′ to 5′ proofreading function. The enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.

Features of the NxGen M-MuLV Reverse Transcriptase

Unit Concentration	200,000 U/mL
Purity (SDS-PAGE)	>99%
SS Exonuclease	2,000 U <5.0% released
DS Exonuclease	2,000 U <0.5% released
Endonuclease	2,000 U <10% converted
E. coli 16S rDNA Contamination	2000 U <10 copies

Unit Definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

Material available for download
NxGen M-MuLV Reverse Transcriptase manual

Products

Note: product availability depends on country - see product detail page.

Details	Cat number & supplier	Size	Price
NxGen M-MuLV Reverse Transcriptase, 200 U/µL 30222-1 · Lucigen	30222-1 Lucigen	50000 units	£257.00 50000 units view
NxGen RNAse Inhibitor, 40 U/µL 30281-1 · Lucigen	30281-1 Lucigen	10000 units	£356.00 10000 units view

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NxGen M-MuLV Reverse Transcriptase manual