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NxGen Grade M-MuLV Reverse Transcriptase

The NxGen® M-MuLV Reverse Transcriptase from LGC Biosearch (Lucigen) is a highly-pure, RNA-dependent DNA polymerase which shows no measurable 3′ to 5′ proofreading function. The enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.

Features of the NxGen M-MuLV Reverse Transcriptase

Unit Concentration 200,000 U/mL
Purity (SDS-PAGE) >99%
SS Exonuclease 2,000 U <5.0% released
DS Exonuclease 2,000 U <0.5% released
Endonuclease 2,000 U <10% converted
E. coli 16S rDNA Contamination 2000 U <10 copies


Unit Definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

Material available for download
NxGen M-MuLV Reverse Transcriptase manual

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
NxGen® M-MuLV Reverse Transcriptase 30222-1 · Lucigen
30222-1
Lucigen
50000 units £171.00
50000 units
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NxGen® M-MuLV Reverse Transcriptase 30222-2 · Lucigen
30222-2
Lucigen
250000 units £692.00
250000 units
view
NxGen® RNAse Inhibitor 30281-1 · Lucigen
30281-1
Lucigen
10000 units £157.00
10000 units
view
NxGen® RNAse Inhibitor 30281-2 · Lucigen
30281-2
Lucigen
50000 units £639.00
50000 units
view