The NanoEX™ system from Aopia Biosciences, is a fully automated platform purpose-built for isolating extracellular vesicles (EVs), lipid nanoparticles (LNPs), gene therapy vectors, and other biological nanoparticles. It delivers unmatched yield and purity, achieving recovery rates of over 90% and removing more than 99.9 % of free proteins, all with less than five minutes of hands-on time.
Asymmetric Nanopore Membrane (ANM) Technology
Aopia’s patented ANM employs a highly uniform and asymmetric pore design that prevents clogging, minimises shear forces, and ensures preservation of EV integrity. By combining high-efficiency bio-nanoparticle capture with advanced purifying action, ANM enables simultaneous concentration and enrichment, outperforming conventional ultrafiltration or tangential flow filtration approaches.
|
|
|
Key Features
- Fully automated operation with under 5 minutes of hands-on time
- High-performance yields: > 90% recovery across EV, LNP, and viral vectors
- Superior purity: > 99.9% removal of free proteins
- Gentle processing to preserve structural and functional integrity of particles
- High scalability: handles sample inputs up to 2L for pilot and therapeutic workflows
- Enclosed single-use cassette format avoids cross-contamination. Cassette options support volumes from research to industrial scales .
| Method | Yield | Purity | Scalability | Automation |
|---|---|---|---|---|
| Ultracentrifugation (UC) | ++ | ++ | ++ | No |
| Polymer precipitation | ++++ | + | ++ | No |
| Immunoaffinity | + | ++++ | + | No |
| Microfluidics | + | +++ | + | No |
| Ultrafiltration | +++ | + | ++ | No |
| Size exclusion chromatography | +++ | +++ | + | Yes |
| Tangential flow filtration | ++++ | ++ | ++++ | No |
| NanoEX | ++++ | ++++ | +++ | Yes |
Advantages & Benefits
- Reproducible performance thanks to automation and pre-programmed protocols
- High yield & purity deliver EV preps that retain structural integrity and functional activity
- Time efficiency: processes up to 250 ml within a few hours, compared to multi-step ultracentrifugation
- Scalable workflow: from biomarker discovery to clinical production, it supports large volume runs (>10L potential via ANM technology)
- Low contamination risk: closed cassette system with waste containment prevents cross-sample carryover
- Minimal shear stress: asymmetric membrane architecture minimises damage to EVs and aggregation of proteins
Material for download
NanoEX sample preparation guide
Products
Note: product availability depends on country - see product detail page.
