Lipid nanoparticles and reagent kits: LipidLaunch

Cayman Chemical specialise in aiding lipid nanoparticle (LNP) expeditions with a wide range of tools available. From preformulated and research-ready LNPs, to reagent kits designed to enable LNP uptake visualisation in target cells, Cambridge Bioscience supply Cayman Chemical's LipidLaunch™ range in its entirety to support all levels of LNP research for the advancement of targeted drug and targeted therapy approaches.

Now available
Download the Cayman Currents LNP edition issue for a comprehensive guide on the latest LNP technologies and advancements.

Benefits of using Cayman Chemical's LipidLaunch range:
• Research-ready LNPs
• Two LNP formats depending on research needs
• Easy-to-use & adaptable protocols
• Wide variety of applications covered
• From experts in LNP formulation, characterisation & optimisation

► Preloaded LNPs
Cayman's preformulated LNPs containing reporter mRNA are intended for proof-of-concept experiments to help determine whether SM-102-based LNPs can effectively lead to expression of proteins of interest in target cell types (in vitro or in vivo).

Figure 1: Transfection of cells with mCherry-encapsulating SM-102 particles: Huh7 hepatocytes (left) and A549 lung epithelial cells (right) were incubated with LipidLaunch SM-102 (mCherry) at an RNA concentration of 150 ng/ml for 24 hours in cell culture media with 10% FBS. Cells were stained with 4 uM Hoechst 33342 and imaged using 590 LED/Texas Red™ and 365 LED/DAPI cubes on an imaging plate reader.

► Loadable LNPs
Cayman's LipidLaunch Loadable LNP SM-102 Kit provides cargo-ready, lyophilised LNPs while designed to facilitate easy and efficient RNA transfection with reduced toxicity when compared to traditional methods.

Figure 2: Loadable LNPs protocol summary: Following rehydration, loadable LNPs are mixed with RNA cargo before the addition of cell-based assay buffer to promote encapsulation. The loaded LNPs are then ready for downstream delivery to target cells.

► LNP Exploration Kits
Cayman’s LipidLaunch LNP-102 Exploration Kit provides a starting point for researchers to explore the feasibility of using SM-102-based LNPs for specific applications without any requirement for specialised equipment.

Figure 3: Firefly luciferase mRNA encapsulated in LNP-102, LNP-0315 and LNP-MC3 injected subcutaneously into CD-1 IGS mice: In vivo luminescence with intraperitoneal injection of D-luciferin) captured by IVIS 8 hours later.

► LNP Uptake Kits
LNP uptake kits enable evaluation of LNP uptake into your cells of choice, using sterol-based fluorescent tracers. Also compatible with other biological readouts.

Figure 4: LNP-102 uptake in hepatocytes: LNP-102 was formulated using a FRR of 3 and a TFR of 15 ml/min. After dialysis against PBS, LNPs were diluted 1:200 in cell culture media and added to Huh7 hepatocytes for 48 hours. Imaging was carried out in PBS on an imaging plate reader at 20X with a GFP LED/filter cube.

Applications
• Study the LNP transfection efficiency in target cells using reporter gene mRNA-LNPs
• Encapsulate your cargo of interest using preformulated LNPs
• Prepare LNPs without specialised equipment
• Visualise the LNP uptake into your target cell type using fluorescence imaging

Materials available for download
Cayman Currents LNP issue
LipidLaunch SM-102 Preloaded LNP insert
LipidLaunch SM-102 LNP Loadable LNP insert
LipidLaunch LNP-102 Exploration Kit insert
LipidLaunch LNP-102 Uptake Kit insert
LNP efficacy application note