Innovative detergents for membrane protein extraction

These innovative calixarene surfactants enable the purification of functional membrane proteins. Requiring no folding, mutagenesis, truncation or fusion and offering a purification method that maintains the functional and structural integrity of membrane proteins, these detergents are the ideal solution for the extraction and stabilisation of membrane proteins.

Benefits of using these detergents
• Membrane proteins extracted and stabilised
• Functional and structural integrity maintained
• No refolding, mutagenesis, truncation, or fusion requirements
• Native state of membrane proteins unaffected

Cayman detergents

Simulation of multidrug efflux pump subunit AcrB (E. coli strain K12) (PDB 5YIL) and adenosine A2A receptor (PDB 5WF5) being extracted from a lipid membrane bilayer and held in active conformations using calixarene-based detergents.

Binding of radioligand on target protein, purified in reference detergent with or without CALXGLUK as an additive

CALXGLUK Graph

Purified protein was incubated with radioligand in absence (total, blue bars) or presence (Non Specific Signal, red bars) of an excess of cold ligand. After filtration on GF/C membranes and washing, scintillation agent was added and radioactivity was detected using a Microbeta2. Specific radioactivity (green bars) corresponds to (total signal) – (non-specific signal).

Material available for download
CALXGLUK product information
CALXCHOL product information
CALX8 product information

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
CALX8 CAY24777-50 mg · Cayman Chemical
CAY24777-50 mg
Cayman Chemical
50 mg £2028.00
50 mg
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CALX8 CAY24777-100 mg · Cayman Chemical
CAY24777-100 mg
Cayman Chemical
100 mg £3244.00
100 mg
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CALX8 CAY24777-250 mg · Cayman Chemical
CAY24777-250 mg
Cayman Chemical
250 mg £5069.00
250 mg
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CALXCHOL CAY24783-10 mg · Cayman Chemical
CAY24783-10 mg
Cayman Chemical
10 mg £641.00
10 mg
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CALXCHOL CAY24783-50 mg · Cayman Chemical
CAY24783-50 mg
Cayman Chemical
50 mg £1923.00
50 mg
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CALXGLUK CAY24784-10 mg · Cayman Chemical
CAY24784-10 mg
Cayman Chemical
10 mg £766.00
10 mg
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CALXGLUK CAY24784-50 mg · Cayman Chemical
CAY24784-50 mg
Cayman Chemical
50 mg £2870.00
50 mg
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CALXGLUK CAY24784-100 mg · Cayman Chemical
CAY24784-100 mg
Cayman Chemical
100 mg £4210.00
100 mg
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