Innovative detergents for membrane protein extraction

These innovative calixarene surfactants enable the purification of functional membrane proteins. Requiring no folding, mutagenesis, truncation or fusion and offering a purification method that maintains the functional and structural integrity of membrane proteins, these detergents are the ideal solution for the extraction and stabilisation of membrane proteins.

Benefits of using these detergents
• Membrane proteins extracted and stabilised
• Functional and structural integrity maintained
• No refolding, mutagenesis, truncation, or fusion requirements
• Native state of membrane proteins unaffected

Simulation of multidrug efflux pump subunit AcrB (E. coli strain K12) (PDB 5YIL) and adenosine A2A receptor (PDB 5WF5) being extracted from a lipid membrane bilayer and held in active conformations using calixarene-based detergents.

Binding of radioligand on target protein, purified in reference detergent with or without CALXGLUK as an additive

Purified protein was incubated with radioligand in absence (total, blue bars) or presence (Non Specific Signal, red bars) of an excess of cold ligand. After filtration on GF/C membranes and washing, scintillation agent was added and radioactivity was detected using a Microbeta2. Specific radioactivity (green bars) corresponds to (total signal) – (non-specific signal).

Material available for download
CALXGLUK product information
CALXCHOL product information
CALX8 product information