DNA methylation ELISA

The Cayman Chemical competitive DNA methylation ELISA enables the accurate quantification of 5-methyl-2'-deoxy cytidine in urine, culture supernatants, plasma, and other sample matrices.

Global changes in methylation can be quantified by measuring plasma or urinary levels of 5-methyl-2'-deoxy cytidine. These changes in methylation can provide valuable information about cancer status of an individual.

Benefits of using the DNA methylation ELISA kit
• Simple procedure
• Wide sample compatibility
• Uses the AChE technology
• Convenient - no 'stop' reagent required
• Highly stable tracer with wide pH range

Features
• Measure 5-methyl-2’-deoxy cytidine in urine, plasma, cell lysates, and tissue culture supernatants
• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure 5-methyl-2’-deoxy cytidine levels down to 3 ng/ml
• Incubation: 18 hours. Development: 90-120 minutes. Read: Colourimetric at 405-420 nm

DNA methylation ELISA kit curve

Why choose a Cayman Chemical assay?
Measure with confidence. Sensitivity defined.

Material available for download
DNA methylation ELISA kit protocol