Frozen human cord blood cells

ZenBio offers a range of frozen human cord blood cells, from haematopoietic stem/progenitor cells (e.g. CD34+) to Umbilical Vein Endothelial Cells (HUVEC).

Cord blood CD34+ progenitor cells are in a phenotypically undifferentiated state. HUVECs are individually processed (or pooled for mixed products) to isolate endothelial cells through collagenase digestion and culture. Frozen HUVEC products are cryopreserved at the end of the primary culture.

Benefits of using these human cord blood cells
• Cells are isolated by immuno-magnetic separation and Ficoll-Paque purification
• Cells are cryopreserved in serum-free medium immediately after isolation
• Samples consented for genetic analysis and commercial research purposes
• Lot QC tested for surface marker characterisation
• Guaranteed purity of 90%
• Viability of >80%
• Serology screening of cells
• Collection and banking conforms and complies with IRB/FDA validated protocols

Cord blood cell pathogen testing
All cryopreserved cord blood cells are tested negative for HIV-1, HIV-2, HTLV I, HTLV II, Syphilis, CMV, HBV and HCV via PCR. However, no known test can offer complete assurance that the cells are pathogen free. Products are tested and are free from mycoplasma contamination. Proper precautions and biological containment should be taken when handling cells of human origin, due to their potential biohazardous nature. All human based products should be handled at a BSL-2 (Biosafety Level 2) or higher.

To prevent clumping and to maximise viability upon thawing, we strongly suggest the use of our Lymphocyte Medium (LYMPH1, LYMPH-1-50) to thaw these cells, especially if you are using a homemade formulation and not achieving success. For HUVEC, ZenBio guarantees cells to reach 15 population doublings when cultured with the Endothelial Growth Medium (ECGM-1).

To request a quote or find out more, please contact our ZenBio specialist.

These products are for use in Biomedical Research only. They are not to be used for any form of human treatment, or for any purpose that is prohibited by law, including any use for human reproductive cloning. It is your responsibility to ensure that you have necessary licensing and ethics approvals in place for the use and storage of any human tissue related material supplied by Cambridge Bioscience.