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EZ DNA Methylation Kits

The EZ DNA Methylation Kit from Zymo Research features a simplified procedure to streamline the bisulphite treatment of DNA.

Based on a three-step reaction that takes place between cytosine and sodium bisulphite, this kit utilises innovative desulphonation technologies to eliminate cumbersome precipitations. Template degradation is reduced and DNA loss is minimised during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including library preparation for next generation sequencing, PCR amplification, endonuclease digestion and microarrays.

The EZ DNA Methylation Kit is recommended for use with the Illumina® Infinium Methylation EPIC BeadChip array.

Benefits of using EZ DNA Methylation kits
• >99% conversion
• >80% recovery
• Elute in as little as 10 µl
• 500 pg - 2 µg DNA input
• 12-16 hours processing time

High throughput requirements
For higher throughput requirements, the spin columns are also available in shallow or deep well 96 well plate format. The two catalogue numbers, D5003 and D5004, are used to differentiate between the binding plates that are included in the kit. Deep and shallow-well binding plates are available to accommodate most rotors and microplate carriers.

DNA binding plate comparison
Binding plate Silicon-A plate Zymo-Spin I-96 plate
Style Shallow well Deep well
Height of plate 19 mm 35 mm
Binding plate/collection plate assembly 43 mm 60 mm
Binding cap/minimum elution volume 5 µg/30 µl 5 µg/15 µl
Cat number D5003 D5004

EZ DNA Methylation Kit comparison
Catalogue number Product Format Elution volume Input
D5001 EZ-96 DNA Methylation Kit Spin-column ≥ 10 µl 500 pg - 2 µg of DNA
D5003 EZ-96 DNA Methylation Kit 96-well (shallow) ≥ 30 µl 500 pg - 2 µg of DNA
D5004 EZ-96 DNA Methylation Kit 96-well (deep) ≥ 15 µl 500 pg - 2 µg of DNA
D5040 EZ-96 DNA Methylation Kit MagPrep Magnetic beads ≥ 25 µl 500 pg - 2 µg of DNA

Material available for download
EZ DNA Methylation Kit manual
Bisulphite conversion for Illumina methylation arrays application note

Illumina is a registered trademark of Illumina


Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
EZ DNA Methylation™ Kit D5001 · Zymo Research
Zymo Research
50 reactions £191.00
50 reactions
EZ DNA Methylation™ Kit D5002 · Zymo Research
Zymo Research
200 reactions £657.00
200 reactions