The EZ DNA Methylation Kit from Zymo Research features a simplified procedure to streamline the bisulphite treatment of DNA.
Based on a three-step reaction that takes place between cytosine and sodium bisulphite, this kit utilises innovative desulphonation technologies to eliminate cumbersome precipitations. Template degradation is reduced and DNA loss is minimised during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including library preparation for next generation sequencing, PCR amplification, endonuclease digestion and microarrays.
The EZ DNA Methylation Kit is recommended for use with the Illumina® Infinium Methylation EPIC BeadChip array.
Benefits of using EZ DNA Methylation kits
• >99% conversion
• >80% recovery
• Elute in as little as 10 µl
• 500 pg - 2 µg DNA input
• 12-16 hours processing time
High throughput requirements
For higher throughput requirements, the spin columns are also available in shallow or deep well 96 well plate format. The two catalogue numbers, D5003 and D5004, are used to differentiate between the binding plates that are included in the kit. Deep and shallow-well binding plates are available to accommodate most rotors and microplate carriers.
| Binding plate | Silicon-A plate | Zymo-Spin I-96 plate |
|---|---|---|
| Style | Shallow well | Deep well |
| Height of plate | 19 mm | 35 mm |
| Binding plate/collection plate assembly | 43 mm | 60 mm |
| Binding cap/minimum elution volume | 5 µg/30 µl | 5 µg/15 µl |
| Cat number | D5003 | D5004 |
EZ DNA Methylation Kit comparison
| Catalogue number | Product | Format | Elution volume | Input |
|---|---|---|---|---|
| D5001 | EZ-96 DNA Methylation Kit | Spin-column | ≥ 10 µl | 500 pg - 2 µg of DNA |
| D5003 | EZ-96 DNA Methylation Kit | 96-well (shallow) | ≥ 30 µl | 500 pg - 2 µg of DNA |
| D5004 | EZ-96 DNA Methylation Kit | 96-well (deep) | ≥ 15 µl | 500 pg - 2 µg of DNA |
| D5040 | EZ-96 DNA Methylation Kit MagPrep | Magnetic beads | ≥ 25 µl | 500 pg - 2 µg of DNA |
Material available for download
EZ DNA Methylation Kit manual
Bisulphite conversion for Illumina methylation arrays application note
Illumina is a registered trademark of Illumina
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| EZ DNA Methylation™ Kit D5001 · Zymo Research | D5001 Zymo Research |
50 reactions |
£191.00
50 reactions
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| EZ DNA Methylation™ Kit D5002 · Zymo Research | D5002 Zymo Research |
200 reactions |
£657.00
200 reactions
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