EZ DNA Methylation-Gold Kits

The EZ DNA Methylation-Gold™ Kits from Zymo Research provide rapid bisulphite conversion. Consolidating DNA denaturation and bisulphite conversion into one step by using temperature denaturation instead of chemical denaturation this kit enables bisulphite conversion in just a 3 hour processing time.

Bisulphite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, next generation sequencing and microarrays.

Benefits of using the EZ DNA Methylation Gold Kits
• > 99% conversion
• 75% recovery
• Elute in as little as 10 µl
• 500 pg - 2 µg input DNA
• 3 hours processing time

Salmon sperm DNA were spiked with fully (CpG) methylated, linearlized pUC19 and pOX plasmid (1.5% w/w) and subjected to bisulfite conversion using the EZ DNA Methylation-Gold kit. Two amplicons, 453 bp and 314 bp, were successfully amplified from the bisulfite converted pUC19 and pOX plasmid DNA, repectively. The amplicons were analyzed in a 2.0% (w/v) TAE/agarose/EtBr gel. The size marker “M” is a 100 bp ladder.

High throughput requirements
For higher throughput requirements, the spin columns are also available in shallow or deep well 96 well plate format. The two catalogue numbers, D5007 and D5008, are used to differentiate between the binding plates that are included in the kit. Deep and shallow-well binding plates are available to accommodate most rotors and microplate carriers.

The automation friendly, Tecan compatible format,  EZ-DNA Methylation-Gold MagPrep, is also available.

Material available for download
EZ DNA Methylation Gold manual

EZ DNA Methylation Gold citations