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Exosome targeting technology: XStamp

The XStamp™ exosome targeting technology from System Biosciences is a patented exosome surface display technology which can be used to place cellular "addresses" on exosomes in order to send them to specific destinations for cargo delivery. Based on a C-terminal fusion of the C1C2 domain from MFG-E8, protein sequences fused to the XStamp tag will display the protein ligand fusion on the surfaces of secreted exosomes, enabling specific delivery.

Benefits of using XStamp exosome targeting technology
• Protein of choice displayed on secreted exosome surface
• Target specific cellular destinations
• Create stable cell lines producing XStamp exosomes

XStamp system
xstamp_system
The protein ligand chosen to display on exosomes is cloned into the MCS and fused to the C1C2 domain. The XStamp lentivector also features a downstream EF1-Puromycin cassette for selection and stable cell line development.

MFG-E8 localises to exosome surfaces (C1C2 domain)
 
xstamp

Fluorescently-labelled antibodies for MFG-E8, CD58 and CD81 were used in combination for FACs analysis. CD58 is a known cell surface marker that is absent on exosomes. CD81 is known to be present both in cells and exosomes. The FACs data show that MFG-E8 is exclusively detected on exosomes and not present in the cells. 

NCAM-XStamp and brain homing peptide 1 for neural targeting
 
xstamp+nacm


NCAM-XStamp construct (cat no. XSTP721PA-1) and BHP1-XStamp construct (cat no. XSTP722PA-1) were transfected separately into mouse MSCs with SBI's XPack-GFP construct (cat no. XPAK530PA-1) for fluorescent tracking. After 48 h, the exosomes were collected. Equal amounts (100 ug) of control (No XStamp), NCAM-XStamp or BHP1-XStamp MSC exosomes were added to Neuro2a neuroblastoma cells in culture. Neurons were imaged for phase and GFP signals after 24 h incubation with the various exosomes to monitor GFP delivery mediated by the XStamp ligand coats.

XStamp with motilin specifically targets exosomes to GI cells

XStamp with motilin specifically targets exosomes to GI cells

Motilin is a 22-amino acid polypeptide hormone that binds to the motilin receptor, which is exclusively expressed in the intestine. XStamp with motilin can be used to target exosomes to GI cells. METHODS: The XStamp-Motilin construct (Cat.# XSTP720PA-1) was transfected into HEK293 cells and after 48 hours, the exosomes were isoalated using ExoQuick-TC. The next day, the XStamp-Motilin exosomes were Exo-Fected with a Texas-Red-labeled siRNA to monitor exosome docking and delivery. The transfected XStamp-Motilin exosomes were then added to MDA-MB-231 Breast Cancer Cells (motilin receptor negative) and to HT-29 Colon Cancer Cells (motilin receptor positive). The cells were imaged after 24 hours for uptake of the Texas-Red-labeled siRNA delivery from the XStamped exosomes. The HT-29 colon cancer cells that are motilin receptor positive took up the XStamp-Motilin exosomes at a much higher rate than the MDA-MB-231 Breast Cancer (motilin receptor negative) cells.

Material available for download
XStamp exosome targeting technology datasheet
XStamp exosome targeting technology manual

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro XSTP710PA-1 · System Biosciences
XSTP710PA-1
System Biosciences
10 µg £665.00
10 µg
view
XStamp MLN exosome Motilin receptor GI tract targeting lentivector XSTP720PA-1 · System Biosciences
XSTP720PA-1
System Biosciences
10 µg £691.00
10 µg
view
XStamp NCAM exosome brain targeting lentivector XSTP721PA-1 · System Biosciences
XSTP721PA-1
System Biosciences
10 µg £691.00
10 µg
view
XStamp BHP1 exosome brain homing peptide targeting lentivector XSTP722PA-1 · System Biosciences
XSTP722PA-1
System Biosciences
10 µg £691.00
10 µg
view
XStamp GE11 exosome EGFR targeting lentivector XSTP723PA-1 · System Biosciences
XSTP723PA-1
System Biosciences
10 µg £691.00
10 µg
view
XStamp Her2 ligand exosome Her2 receptor targeting lentivector XSTP724PA-1 · System Biosciences
XSTP724PA-1
System Biosciences
10 µg £691.00
10 µg
view
XStamp CD40L exosome CD40 receptor targeting lentivector XSTP725PA-1 · System Biosciences
XSTP725PA-1
System Biosciences
10 µg £691.00
10 µg
view
XStamp IL2 exosome IL-2 receptor targeting lentivector XSTP726PA-1 · System Biosciences
XSTP726PA-1
System Biosciences
10 µg £691.00
10 µg
view