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Exosome protein engineering: XPack protein packaging vectors

The XPack™ technology from System Biosciences enables cell-mediated generation of ready-to-use exosomes packaged with any protein of choice. Incorporating an optimised ‘XPack’ peptide sequence within the XPack cloning and expression lentivectors, your protein of interest is targeted to the interior exosomal membrane within a producer cell line. These engineered exosomes can then be easily isolated and delivered to target cells.

Benefits of using the XPack technology
•    Two promoter options for expression in a wide range of cells
•    Comes with a choice of three reporters (GFP, RFP, luciferase)
•    Bioluminescent exosome generation possible
•    Ideal for exosome labelling, cargo tracking and stable cell line generation
•    XPack peptide tag for robust exosome targeting

Pack-CDKN1B exosomes reduce cellular proliferatio

SBI cloned the CDKN1B gene into CMV-XP-MCS-EF1α-Puro Cloning Lentivector (Cat.# XPAK510PA-1), transfected this construct into HEK293 cells, and isolated exosomes after 48 hours using ExoQuick-TC®. SBI then added 5, 50, or 100 µg of XPack-CDKN1B exosomes to approximately 5000 HEK-293 target cells in 96-well tissue culture plates at 24-hour intervals over the course of 72 hours. Exosomes isolated from non-transfected cells were used for controls. Cellular proliferation was quantified using an MTT Assay (Millipore catalog # CT02) and normalised to HEK-293 cells treated with control exosomes. XPack-CDKN1B exosomes consistently reduce the rate of cellular proliferation. 

XPack efficiently packages GFP into exosomes

XPack efficiently packages GFP into exosomes. (

(Upper panel) XPack-GFP localisation during packaging. (Lower panel) Western blot showing robust loading of GFP into the isolated exosomes. 

Material available for download
XPack product sheet
XPack user manual

XPack technical references & citations

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector XPAK510PA-1 · System Biosciences
XPAK510PA-1
System Biosciences
10 µg £665.00
10 µg
view
XPack CMV-XP-GFP-EF1-Puro Expression Lentivector XPAK530PA-1 · System Biosciences
XPAK530PA-1
System Biosciences
10 µg £691.00
10 µg
view
XPack CMV-XP-RFP-EF1-Puro Expression Lentivector XPAK531PA-1 · System Biosciences
XPAK531PA-1
System Biosciences
10 µg £691.00
10 µg
view
XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivector XPAK532PA-1 · System Biosciences
XPAK532PA-1
System Biosciences
10 µg £691.00
10 µg
view
XPack MSCV-XP-MCS-EF1-Puro Cloning Lentivector XPAK710PA-1 · System Biosciences
XPAK710PA-1
System Biosciences
10 µg £665.00
10 µg
view
XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector XPAK730PA-1 · System Biosciences
XPAK730PA-1
System Biosciences
10 µg £691.00
10 µg
view
XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector XPAK732PA-1 · System Biosciences
XPAK732PA-1
System Biosciences
10 µg £691.00
10 µg
view
XPack CMV-XP-GFP-EF1-Puro Expression Lentivirus XPAK530VA-1 · System Biosciences
XPAK530VA-1
System Biosciences
>1 million IFU £691.00
>1 million IFU
view
XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivirus XPAK532VA-1 · System Biosciences
XPAK532VA-1
System Biosciences
>1 million IFU £691.00
>1 million IFU
view
XPack CMV-XP-GFP-EF1-Puro Stable HEK293 Producer Cell Line XPAK530CL-1 · System Biosciences
XPAK530CL-1
System Biosciences
2 million cells £1197.00
2 million cells
view
XPack CMV-XP-Luciferase-EF1-PuroStable HEK293 Producer Cell Line XPAK532CL-1 · System Biosciences
XPAK532CL-1
System Biosciences
2 million cells £1197.00
2 million cells
view
XPack MSCV-XP-GFP-EF1-Puro Expression Lentivirus XPAK730VA-1 · System Biosciences
XPAK730VA-1
System Biosciences
>1 million IFU £691.00
>1 million IFU
view
XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivirus XPAK732VA-1 · System Biosciences
XPAK732VA-1
System Biosciences
>1 million IFU £691.00
>1 million IFU
view
XPack-GFP loaded HEK293 exosomes XPAK100EX-G · System Biosciences
XPAK100EX-G
System Biosciences
50 µg exosomes £586.00
50 µg exosomes
view