96 sample exosome purification kit: Exo-spin 96

Exo-spin™ 96 from Cell Guidance Systems enables exosome isolation from up to 96 individual samples in less than 30 minutes. Utlising size exclusion chromatography (SEC) technology and a detachable 8 x 12 well strip format, 8-96 samples can be processed simultaneously at the benchtop, without the need for instrumentation. This kit can be used to isolate exosomes directly from protein-rich samples, such as sera and plasma, and concentrated samples of low-protein biofluids, such as cell culture media and urine.

Benefits of using Exo-spin 96
• 30 minute workflow
• Outstanding reproducibility
• Benchtop process - no instrumentation required
• Flexible 8 x 12 well detachable strip format
• Convenience of purified exosomes eluted directly into a 96 well plate
• Compatible with protein-rich samples eg sera, plasma & concentrated samples of low-protein biofluids eg cell culture media, urine etc

Exo-spin isolated exosomes characterised with NTA

Excellent reproducibility is routinely achieved with Exo-Spin. Data for 8 columns shown. Exosomes have been isolated using Exo-spin 96 and analysis performed with the ZetaView® instrument.

Exo-spin comparitive data

Exo-spin shows superior yield and purity. Data determined by nanoparticle tracking analysis (NTA). Each curve represents the average of 3 technical replicate measurements for each exosome isolation method and biofluid triplicate experiment. (PM = Precipitation Method). Data generated for Exo-spin mini columns which have the same resin and measurements. Adapted from (Martins, TS et al., 2018).

RNA analysis citations
Wang L., Wang C., Jia X. and Yu J. (2018). Circulating Exosomal miR-17 Inhibits the Induction of Regulatory T Cells via Suppressing TGFBR II Expression in Rheumatoid Arthritis. Cellular Physiology and Biochemistry; 50:1754–1763.

Emanueli C., Shearn AI., Laftah A., Fiorentino F., Reeves BC., Beltrami C., Mumford A., Clayton A., Gurney M., Shantikumar S. and Angelini G. (2016). Coronary Artery-Bypass-Graft Surgery Increases the Plasma Concentration of Exosomes Carrying a Cargo of Cardiac MicroRNAs: An Example of Exosome Trafficking Out of the Human Heart with Potential for Cardiac Biomarker Discovery. PLoS One 29;11(4):e0154274.

Mass spectrometry citations
Menezes-Neto A., Fidalgo Sáez MJ., Lozano-Ramos I., Segui-Barber J., Martin-Jaular L., Estanyol Ullate JM., Fernandez-Becerra C., Borrás FE., and del Portillo HA. (2015). Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals. Journal of Extracellular Vesicles; 4: 10.3402/jev.v4.27378.

Functional study citations
Sheller-Miller S, Trivedi J, Yellon SM, and Menon R. (2019). Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Scientific Reports 24;9(1):608.

Material available to download
Exo-spin 96 user guide

ZetaView is a registered trademark of Dr. Hanno Wachernig