Monitor exosome cargo delivery in real-time: ExoGlow

The ExoGlow™ kit from System Biosciences enables the tracking of cellular interaction and uptake of exosomes with low background and high selectivity. With proprietary fluorescent dyes which improve your ability to track and localise extracellular vesicles (EVs) specifically and covalently labelling internal EV proteins, EV mRNAs and EV membranes to monitor exosome cargo deliver in real time. Monitor EV trafficking in vivo with the ExoGlow-Vivo EV Labeling Kit (Near IR), a unique dye developed for following EVs in animal models and tissues.

Benefits of using ExoGlow
• Simple protocol 
• Real-time tracking of cellular interaction & uptake 
• Robust signal specific for internal EV proteins, mRNA & membranes 
• Low sample input
• In vivo kit optimised for imaging EVs in animal models 
• NTA kit that specifically labels EVs for fluorescent NTA quantitation

ExoGlow-Protein enables clear visualisation of labelled EVs being internalised by target cells

SBI labelled HEK293T EVs with ExoGlow-Protein and followed uptake by HEK293T cells. The punctate fluorescence signal shows internalisation of labelled EVs by the target cells. 

ExoGlow-Membrane is specific for intact membranes

PBS, liposomes, liposomes pre-solubilised with detergent (0.05% Tx100), EVs, or lipoproteins were incubated. Only the samples with intact membranes—liposomes and EVs (blue)—show a high fluorescence signal, demonstrating ExoGlow-Membrane’s specificity for intact membranes. 

Material available for download
Exo-Glow Protein EV Labeling Kits user manual
ExoGlow-Membrane EV Labelling Kit user manual
ExoGlow-RNA EV Labelling Kit user manual
ExoGlow-Vivo EV Labelling Kit (Near IR) user manual
ExoGlow-NTA (for Particle Metrix ZetaView) user manual
ExoGlow-NTA (for Malvern NanoSight) user manual