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Exosome transfection reagent: Exo-Fect

The Exo-Fect™ reagent enables the high efficiency transfection of nucleic acids directly into isolated exosomes. In less than one hour, create novel exosome delivery vehicles for siRNA, miRNA, mRNA, plasmid DNA and even small molecules for transport into target cells.

Benefits of using Exo-Fect
• Simple protocol
• No electroporation required
• Load siRNA, miRNA, mRNA and plasmid DNA
• Load small molecules for drug discovery applications
• Texas Red-labelled positive control included

Exo-Fect efficiently loads small RNAs into exosomes for delivery to recipient cells

Exo-Fect efficiently loads siRNA into exosomes

Fluorescence imaging shows exosomes that were loaded with either a Texas Red end-labelled siRNA (left panels), or unlabelled siRNA (right panels) using Exo-Fect, and then immobilised onto Exo-Flow CD63 magnetic beads.

Exo-Fect transfected exosomes deliver siRNA cargo to recipient cells.

Exo-Fect transfected exosomes deliver siRNA cargo to recipient cells

The siRNA-loaded exosomes from above were eluted from the Exo-Flow CD63 magnetic beads and then added to HEK293 cells. Fluorescence of the recipient cells indicates that the Texas Red-labeled siRNA cargo was successfully delivered.

Transfection of mRNAs and plasmid DNA directly into exosomes

Left panels: 1 µg of mRNA encoding RFP was transfected into exosomes. These exosomes were added to HEK293 cells and imaged for RFP protein production after 24 h. Right panels: 5 µg of plasmid DNA encoding a GFP gene was transfected into exosomes and added to HEK293 cells. The cells were imaged for GFP protein presence after 48 h.

Working with hard-to-transfect cells?
We also offer an easy-to-use exosome-based kit for the delivery of nucleic acids including siRNA, miRNAs, mRNAs & plasmid DNA to a variety of hard-to-transfect cells. Find out more.

Material available for download
Exo-Fect Transfection Kit protocol
Exo-Fect siRNA/miRNA Transfection Kit user manual

Exo-fect citations

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Exo-Fect Exosome Transfection Kit EXFT10A-1 · System Biosciences
EXFT10A-1
System Biosciences
10 reactions £217.00
10 reactions
view
Exo-Fect Exosome Transfection Kit EXFT20A-1 · System Biosciences
EXFT20A-1
System Biosciences
20 reactions £391.00
20 reactions
view
MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes EXOP-100A-1 · System Biosciences
EXOP-100A-1
System Biosciences
50 µg £372.00
50 µg
view
MDA-MB-231 Human breast cancer, aggressive/invasive/metastatic cell line: >1x10^10 frozen exosomes EXOP-105A-1 · System Biosciences
EXOP-105A-1
System Biosciences
50 µg £372.00
50 µg
view
HEK293 Human embryonic kidney cell line: >1x10^10 frozen exosomes EXOP-110A-1 · System Biosciences
EXOP-110A-1
System Biosciences
50 µg £372.00
50 µg
view
PC-3 Human prostate cancer cells derived from metastatic cancer cell line: >1x10^10 frozen exosomes EXOP-115A-1 · System Biosciences
EXOP-115A-1
System Biosciences
50 µg £372.00
50 µg
view
A549 Human non-small cell lung cancer cell line: >1x10^10 frozen exosomes EXOP-120A-1 · System Biosciences
EXOP-120A-1
System Biosciences
50 µg £372.00
50 µg
view
H841 Human small cell lung cancer cell line: >1x10^10 frozen exosomes EXOP-125A-1 · System Biosciences
EXOP-125A-1
System Biosciences
50 µg £372.00
50 µg
view
H196 Human small cell lung cancer cell line: >1x10^10 frozen exosomes EXOP-130A-1 · System Biosciences
EXOP-130A-1
System Biosciences
50 µg £372.00
50 µg
view
DMS114 Human small cell lung cancer cell line: >1x10^10 frozen exosomes EXOP-135A-1 · System Biosciences
EXOP-135A-1
System Biosciences
50 µg £372.00
50 µg
view
PCS-500-011 Human pre-adipose derived mesenchymal stem cell: >1x10^10 frozen exosomes EXOP-140A-1 · System Biosciences
EXOP-140A-1
System Biosciences
50 µg £479.00
50 µg
view
PCS-500-012 Human bone marrow-derived mesenchymal stem cell line: >1x10^10 frozen exosomes EXOP-145A-1 · System Biosciences
EXOP-145A-1
System Biosciences
50 µg £479.00
50 µg
view
JAWSII Mouse bone marrow immature dendritic cell line: >1x10^10 frozen exosomes EXOP-200A-1 · System Biosciences
EXOP-200A-1
System Biosciences
50 µg £479.00
50 µg
view
BC-3 Human B lymphocyte cell line: >1x10^6 frozen exosomes EXOP-150A-1 · System Biosciences
EXOP-150A-1
System Biosciences
50 µg £479.00
50 µg
view
Jurkat Clone E6-1 Human T lymphocyte cell line: >1x10^6 frozen exosomes EXOP-155A-1 · System Biosciences
EXOP-155A-1
System Biosciences
50 µg £479.00
50 µg
view
JM1 Human T pre-B lymphoblast cell line: >1x10^6 frozen exosomes EXOP-160A-1 · System Biosciences
EXOP-160A-1
System Biosciences
50 µg £479.00
50 µg
view
RAWS 264.7 Mouse macrophage cell line: >1x10^6 frozen exosomes EXOP-165A-1 · System Biosciences
EXOP-165A-1
System Biosciences
50 µg £479.00
50 µg
view