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  4. Exosome-depleted FBS: Exo-FBS

Exosome-depleted FBS: Exo-FBS

The Exo-FBS™ exosome depleted FBS growth supplement from System Biosciences enables the study of cellular secreted exosomes in culture, without the concern of contamination. Containing very few CD63-positive bovine exosomes and no detectable bovine microRNAs, Exo-FBS provides comparable cellular growth rates to standard FBS without interference from bovine exosomes. 

Benefits of using Exo-FBS
• Very few CD63-positive bovine exosomes
• No detectable bovine microRNAs
• Robust cellular growth rates supported
• Comparable cellular growth rates to standard FBS
• Same method of use as standard FBS (add 10% in DMEM or RPMI)

Greatly reduced levels of bovine exosomes with Exo-FBS

Greatly Reduced Levels of Bovine Exosomes with Exo-FBS

qPCR assays show undetectable levels of bovine exosomal miRNAs in Exo-FBS. While standard FBS contains amplifiable miRNAs (12 of the 72 individual miRNAs tested, left panels), Exo-FBS shows no amplifiable miRNAs (right panels). Standard FBS and Exo-FBS media supplements (4 ml) were treated with Trizol extraction methods to recover exosomal RNAs. RNA was converted to cDNA and 72 individual bovine microRNAs were measured by qPCR using SBI’s QuantiMir system.
 
Exo-FBS supports more robust cell growth than Company G’s exosome-depleted FBS

HepG2 cells were grown in media containing either standard FBS (control), Exo-FBS, or Company G’s exosome-depleted FBS. Data is shown after one and two passages and illustrates that Exo-FBS provides a higher number of viable cells (left panel, viability assessed using a CCK-8 assay) and a healthier cell morphology (right panel) than Company G’s product.

HepG2 cells were grown in media containing either standard FBS (control), Exo-FBS, or Company G’s exosome-depleted FBS. Data is shown after one and two passages and illustrates that Exo-FBS provides a higher number of viable cells (left panel, viability assessed using a CCK-8 assay) and a healthier cell morphology (right panel) than Company G’s product.

Exo-FBS citations

Material available for download
Exo-FBS user manual

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Exosome-depleted FBS Media Supplement EXO-FBS-50A-1 · System Biosciences
EXO-FBS-50A-1
System Biosciences		 50 ml £202.00
50 ml
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Exosome-depleted FBS Media Supplement EXO-FBS-250A-1 · System Biosciences
EXO-FBS-250A-1
System Biosciences		 250 ml £872.00
250 ml
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