Antibody-based exosome quantification: ExoELISA & ExoELISA-ULTRA

The ExoELISA™ and ExoELISA-ULTRA™ kits from System Biosciences (SBI) enable the sensitive detection and quantification of specific exosomal subpopulations. Compatible with exosomes isolated by ExoQuick, ExoQuick-TC or by ultracentrifugation techniques, the ExoELISA range enables the easy quantification of the three well-characterised exosome markers CD9, CD63 or CD81.

The ExoELISA-ULTRA kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.

Benefits of using ExoELISA
•   Detect as little as 1ug / 500 µg protein equivalent
•   Compatible with all major exosome isolation methods
•   Calibrated internal standards enable quantitation of exosomes carrying CD9, CD63 or CD81

Quantification of exosomes using ExoELISA

The exosome particles and their proteins are directly immobilised onto the wells of the microtiter plate. After binding, wells are coated with a block agent to prevent nonspecific binding of the detection antibody. The detection antibody is added to the wells for binding to specific antigen (e.g. CD63) protein on the exosomes. Extra-sensitive TMB is used for the assay read-out. ExoELISA kits come with exosome standards to make calibration curves.

Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis

Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying CD63 from the ExoELISA data.