The ExoBacteria™ kit from System Biosciences enables isolation of outer membrane vesicles (OMVs) from E. coli and other Gram-negative bacteria with a fast and simple workflow. Using an innovative, precipitation-free gravity column, the ExoBacteria OMV isolation kit enables the harvest of high yield and high purity OMVs from bacterial culture medium in less than one hour.
Benefits of using the ExoBacteria OMV Isolation Kit
• The only kit devoted to isolation of bacterial OMVs
• Twice as fast as ultracentrifugation
• High yield
• High purity
• Easy to setup
• Each kit contains reagents and consumables to process 20 reactions
ExoBacteria OMV Isolation Kit is a better way to isolate OMVs
OMVs isolated using the ExoBacteria OMV isolation kit are similar in appearance, but with tighter distribution than OMVs isolated via ultracentrifugation.
The ExoBacteria OMV isolation kit delivers a narrower size distribution of OMVs and higher yields than ultracentrifugation
(A) Comparison of fluorescent NTA analysis of E. coli-derived bacterial OMVs isolated using either ExoBacteria OMV Isolation Kit (blue line) or ultracentrifugation (grey line) shows how the ExoBacteria™ OMV isolation kit delivers a much narrower size distribution of isolated OMVs than a conventional ultracentrifugation approach. (B) In addition, total yield is 20-fold higher with the ExoBacteria OMV isolation kit (1.38 x 108 particles/µg of protein) compared to ultracentrifugation (0.0657 x108 particles/µg of protein).
Identify more OMV proteins with OMVs isolated using the ExoBacteria OMV Isolation Kit
(A) Comparison of the total number of proteins identified via mass spectrometry from OMV samples isolated using either the ExoBacteria OMV isolation kit or ultracentrifugation shows a wider range of proteins identified from OMVs isolated with the ExoBacteria OMV isolation kit. All but 7 of the proteins identified in the OMVs isolated using ultracentrifugation were also found in the OMVs isolated using the ExoBacteria kit, suggesting that the higher yield and higher purity delivered by the ExoBacteria OMV isolation kit expand your ability to derive insights from OMVs. (B) More of the proteins from the ExoBacteria OMV kit-isolated OMVs were found in the EV-Pedia database than from OMVs isolated using ultracentrifugation, demonstrating the increased specificity of the ExoBacteria OMV isolation kit.
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