The EVeryRNA™ EV RNA purification kits from SBI deliver high yield total extracellular vesicle (EV) RNA, including small RNAs, even with low amounts of input. Highly pure RNA is eluted in a small sample volume, generating a highly concentrated prep and increasing the amount of RNA used in a single downstream reaction. This results in better data-coverage quality and overcomes many of the typical challenges associated with RNA isolation from EVs.
The EVeryRNA EV RNA Purification System can be used with EVs isolated by commonly-used methods, including ExoQuick®, SmartSEC™ and ultracentrifugation, and comes with sufficient reagents to perform 20 purification reactions. EVeryRNA technology is also available bundled with SBI’s powerful EV isolation technologies, as well as a cDNA Synthesis and Pre-amplification Kit.
Benefits of using the EVeryRNA EV RNA Purification System
• High yield exoRNA isolation
• Isolation in <30 minutes
• Achieve phenol-level yields using a safer, column-based method
• Delivers highly-pure RNA using the included DNase I
• Compatible with most downstream applications, including RNA-seq and miRNA profiling
• Use with EVs isolated by commonly-used methods, such as ExoQuick, SmartSEC and ultracentrifugation
The EVeryRNA column-based workflow is easy to implement and can be completed in less than 30 minutes
EVeryRNA EV RNA purification workflow delivers high yields of highly concentrated RNA from already isolated EVs.
EVeryRNA captures everything
The EVeryRNA EV RNA Purification System was used to isolate RNA from 10,000 cells (Lane 1), from EVs that were isolated from 250 µL of serum using SmartSEC Single (Lane 2), and from buffer spiked with 0.1 pmol of Cel-miR-39 (Lane 3). Isolated RNA shown in lane 1 is of high quality with an RNA integrity number (RIN) of 9.9 and a 28S/18S RNA ratio of 1.5. Multiple bands in lane 2 demonstrate that EVeryRNA captures RNAs of different lengths from EVs with no apparent bias or size preference. The strong signal from the spiked-in miRNA in lane 3 demonstrates the good recovery of even small RNAs.
EVeryRNA delivers similar amounts of RNA as phenol-based methods
EVs were isolated from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39 then RNA was isolated using EVeryRNA and a phenol-based kit. The isolated RNA was reverse transcribed using the EVeryRNA cDNA Synthesis & Pre-amplification Kit and the copy number of Cel-miR-39 measured .The EVeryRNA EV RNA Purification System delivered similar levels of Cel-miR-39 as the phenol-based method.
EVeryRNA efficiently isolates longer RNAs like mRNA
The EVeryRNA EV RNA isolation kit was used to isolate mRNA from cells overexpressing eGFP. Robust levels of eGFP mRNA are recovered when cells are overexpressing eGFP.
Material available for download
EVeryRNA EV RNA Purification System manual