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Episomal DNA Vectors: Minicircles

The Minicircle Technology from System Biosciences (SBI) is is a great option for gene expression. Produced as small, excised, circular DNA fragments from a parental plasmid, the non-viral, episomal Minicircle expression cassette is free of any bacterial plasmid DNA sequences and comes with a variety of promoter and reporter combinations. Featuring a smaller size, Minicircles facilitate more efficient transfection than standard-sized plasmids, and although they do not replicate in the host cell, expression lasts for 14 days or longer in dividing cells and can continue for months in non-dividing cells.

Benefits of using Minicircle technology
• Episomal expression sustained over weeks
• Foreign DNA-free
• Unlimited insert size
• Optimised coli minicircle production strain
• Effective in vitro and in vivo
• Choice of promoter, reporter gene and vector format

minicircle technology

Generating minicircle DNA from the parental minicircle plasmid

Generating minicircle DNA from the parental minicircle plasmid

Easy, sustained transfection in most cell types. Transfection of 1 μg of minicircle DNA (pMC.CMV-MCS-EF1-GFPSV40PolyA) into HEK293 cells delivers over one week of robust gene expression.

Please note: Parental minicircle plasmids and the ZYCY10P3S2T Producer Bacterial Strain are available for purchase by not-for-profit researchers only. Commercial users may purchase pre-made, ready-to-transfect minicircle DNA only. SBI also offers custom parental plasmid cloning and minicircle DNA production to both not-for-profit and commercial end users. Please contact our SBI specialists for additional details.

Material available for download
MC-Easy™ Minicircle Production Kit brochure

Minicircle citations

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Pre-made Minicircle CMV-GFP-SV40PolyA (positive control) MN601MC-1 · System Biosciences
MN601MC-1
System Biosciences
40 µg £623.00
40 µg
view
MC-Easy Minicircle DNA Production Kit MN915A-1 · System Biosciences
MN915A-1
System Biosciences
10 preparations £1133.00
10 preparations
view
Pre-made Minicircle CMV-rRFP-SV40PolyA (positive control) MN602MC-1 · System Biosciences
MN602MC-1
System Biosciences
40 µg £623.00
40 µg
view
MC-Easy Minicircle DNA Production Kit MN925A-1 · System Biosciences
MN925A-1
System Biosciences
10 preparations POA
10 preparations
view
Mouse IL-23 pre-made Minicircle DNA [RSV->FLAG-mP40-mP19-pA] MN651MC-1 · System Biosciences
MN651MC-1
System Biosciences
100 µg £788.00
100 µg
view
ZYCY10P3S2T E. coli Minicircle producer strain MN900A-1 · System Biosciences
MN900A-1
System Biosciences
5 vials POA
5 vials
view
MC-Easy Minicircle DNA Production Kit MN910A-1 · System Biosciences
MN910A-1
System Biosciences
5 preparations £610.00
5 preparations
view
MC-Easy Minicircle DNA Production Kit MN920A-1 · System Biosciences
MN920A-1
System Biosciences
5 preparations POA
5 preparations
view
Minicircle Dual Reporter: CMV-RFP-T2A-Luciferase DNA BLIV500MC-1 · System Biosciences
BLIV500MC-1
System Biosciences
30 µg £605.00
30 µg
view
Minicircle Dual Reporter: CMV-RFP-T2A-Luciferase Parental Plasmid BLIV500MN-1 · System Biosciences
BLIV500MN-1
System Biosciences
10 µg £838.00
10 µg
view
Minicircle Dual Reporter: CMV-GFP-T2A-Luciferase DNA BLIV501MC-1 · System Biosciences
BLIV501MC-1
System Biosciences
30 µg £605.00
30 µg
view
Minicircle Dual Reporter: CMV-GFP-T2A-Luciferase Parental Plasmid BLIV501MN-1 · System Biosciences
BLIV501MN-1
System Biosciences
10 µg £838.00
10 µg
view
Minicircle Dual Reporter: EF1-RFP-T2A-Luciferase DNA BLIV502MC-1 · System Biosciences
BLIV502MC-1
System Biosciences
30 µg £605.00
30 µg
view
Minicircle Dual Reporter: EF1-RFP-T2A-Luciferase Parental Plasmid BLIV502MN-1 · System Biosciences
BLIV502MN-1
System Biosciences
10 µg £838.00
10 µg
view
Minicircle Dual Reporter: EF1-GFP-T2A-Luciferase DNA BLIV503MC-1 · System Biosciences
BLIV503MC-1
System Biosciences
30 µg £605.00
30 µg
view
Minicircle Dual Reporter: EF1-GFP-T2A-Luciferase Parental Plasmid BLIV503MN-1 · System Biosciences
BLIV503MN-1
System Biosciences
10 µg £838.00
10 µg
view
Minicircle Dual Reporter: UBC-RFP-T2A-Luciferase DNA BLIV504MC-1 · System Biosciences
BLIV504MC-1
System Biosciences
30 µg £605.00
30 µg
view
Minicircle Dual Reporter: UBC-RFP-T2A-Luciferase Parental Plasmid BLIV504MN-1 · System Biosciences
BLIV504MN-1
System Biosciences
10 µg £838.00
10 µg
view
Minicircle Dual Reporter: UBC-GFP-T2A-Luciferase DNA BLIV505MC-1 · System Biosciences
BLIV505MC-1
System Biosciences
30 µg £605.00
30 µg
view
Minicircle Dual Reporter: UBC-GFP-T2A-Luciferase Parental Plasmid BLIV505MN-1 · System Biosciences
BLIV505MN-1
System Biosciences
10 µg £838.00
10 µg
view
Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase DNA BLIV506MC-1 · System Biosciences
BLIV506MC-1
System Biosciences
30 µg £605.00
30 µg
view
Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase Parental Plasmid BLIV506MN-1 · System Biosciences
BLIV506MN-1
System Biosciences
10 µg £838.00
10 µg
view
Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase DNA BLIV507MC-1 · System Biosciences
BLIV507MC-1
System Biosciences
30 µg £605.00
30 µg
view
Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase Parental Plasmid BLIV507MN-1 · System Biosciences
BLIV507MN-1
System Biosciences
10 µg £838.00
10 µg
view
Minicircle Single Reporter: CMV-Luciferase Minicircle BLIV510MC-1 · System Biosciences
BLIV510MC-1
System Biosciences
30 µg £605.00
30 µg
view