Competent cells for CRISPR GeCKO

The Endura™ competent cells from Lucigen enable the reliable and efficient cloning and maintenance of CRISPR clones. Offering the highest efficiency for GeCKO lentiviral library construction, the Endura competent cells are available in electro and chemically competent formats for high DNA yields and exceptional library diversity.

Benefits of using the Endura competent cells
• Recommended in CRISPR GeCKO library protocols
• Generate lentiviral guide RNA libraries
• Available in electro and chemically competent formats
• Highest efficiency commercially available cells for lentiviral cloning:  > 1 x 10⁷ cfu/µg (chemically competent) or 1 x 10¹⁰ cfu/µg (electrocompetent)

Free samples available
Request an Endura electrocompetent cells (DUO) sample
Request an Endura chemically competent cells (DUO) sample

Transformation efficiencies of electrocompetent clone-stabilising strains

Genotype
Genotype recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (StrR ) xyl-5 λ– leu mtl-1 F – mcrB mrr hsdS20(rB – , mB – )

Material available for download
Endura chemically and electrocompetent cells user manual
Endura chemically and electrocompetent cells flyer
GeCKOv2.0 library amplification protocol

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