High efficiency transfection reagents for CRISPR/Cas9

The range of high efficiency transfection reagents for CRISPR/Cas9 delivery from Mirus Bio are ideal for the efficient transfection of plasmid DNA, mRNA, gRNA and ribonucleoprotein into multiple cell types including hard-to-transfect cells.

Transfection reagents for CRISPR/Cas9 component delivery
• TransIT-X2® - novel polymeric system ideal for delivery of CRISPR/Cas9 components in DNA, gRNA and RNP formats
• TransIT®-mRNA - high efficiency low toxicity reagent designed to deliver CRISPR/Cas9 components in mRNA and gRNA formats.
• Ingenio® - high efficiency electroporation solution compatible with most conventional electroporation devices delivering CRISPR/Cas9 components in DNA, mRNA, gRNA and RNP formats.

	DNA	mRNA	gRNA	RNP
TransIT-X2 dynamic delivery system	ü		ü	ü
TransIT-mRNA transfection kit		ü	ü
Ingenio electroporation solution	ü	ü	ü	ü

DNA, RNA and protein formats for CRISPR genome editing

TransIT-X2 Dynamic Delivery System was used to deliver Cas9 pDNA/gRNA and Cas9 protein/gRNA (RNP complex). TransIT-mRNA was used to deliver Cas9 mRNA/gRNA. A T7E1 mismatch assay was used to measure cleavage efficiency at 48 hours post-transfection. For more details, see our methods for delivery of CRISPR/Cas9 DNA, RNA and RNP.

Efficient genome editing with Cas9 plasmid DNA andguide RNA Oligonucleotides

fficient genome editing with Cas9 plasmid DNA andguide RNA Oligonucleotides

HEK293T/17, U2OS and NHDF cells were co-transfected with 0.5 µg of Cas9 encoding pDNA (MilliporeSigma) and 50nM PPIB targeting 2-part gRNA (Dharmacon) using TransIT-X2® Dynamic Delivery System (2 µl/well of a 24-well plate, Mirus Bio). A T7E1 mismatch detection assay was used to measure cleavage efficiency at 48 hours post-transfection.

Material available for download
Optimisation of DNA, RNA and RNP delivery for efficient mammalian cell engineering
CRISPR brochure
Ingenio electroporation for CRISPR Cas9 RNP delivery
TransIT-mRNA for CRISPR Cas9 mRNA and gRNA delivery
TransIT-X2 for CRISPR Cas9 Plasmid and gRNA delivery
TransIT-X2 for CRISPR Cas9 Plasmid delivery
TransIT-X2 for CRISPR Cas9 RNP and Oligo delivery
TransIT-X2 for CRISPR Cas9 RNP delivery

Free samples
Request a sample of any of these transfection reagents

Amaxa is a registered trademarks of the Lonza Cologne AG in Germany and/or U.S. and/or other countries

Bio-Rad® is a registered trademark of Bio-Rad Laboratories, Inc
BTX® is a registered trademark of Harvard Apparatus

Products

Note: product availability depends on country - see product detail page.

Details	Cat number & supplier	Size	Price
TransIT-mRNA Transfection Kit MIR 2225 · Mirus	MIR 2225 Mirus	0.4 ml	£527.00 0.4 ml view
TransIT-mRNA Transfection Kit MIR 2250 · Mirus	MIR 2250 Mirus	1 ml	£941.00 1 ml view
TransIT-mRNA Transfection Kit MIR 2255 · Mirus	MIR 2255 Mirus	5 x 1 ml	£4112.00 5 x 1 ml view
TransIT-TKO Transfection Reagent MIR 2256 · Mirus	MIR 2256 Mirus	10 x 1.5 ml	£7609.00 10 x 1.5 ml view
Ingenio Electroporation Solution MIR 50111 · Mirus	MIR 50111 Mirus	25 reactions	£236.00 25 reactions view
Ingenio Electroporation Kit for the EZporator Electroporation System and Lonza-Amaxa Nucleofector II/2b devices (0.2 cm cuvettes and cell droppers) MIR 50112 · Mirus	MIR 50112 Mirus	25 reactions	£373.00 25 reactions view
Ingenio Electroporation Solution MIR 50114 · Mirus	MIR 50114 Mirus	50 reactions	£373.00 50 reactions view
Ingenio Electroporation Kit for the EZporator Electroporation System and Lonza-Amaxa Nucleofector II/2b devices (0.2 cm cuvettes and cell droppers) MIR 50115 · Mirus	MIR 50115 Mirus	50 reactions	£651.00 50 reactions view
Ingenio Electroporation Solution MIR 50117 · Mirus	MIR 50117 Mirus	100 reactions	£651.00 100 reactions view
TransIT-X2 Dynamic Delivery System MIR 6000 · Mirus	MIR 6000 Mirus	1.5 ml	£796.00 1.5 ml view
TransIT-X2 Dynamic Delivery System MIR 6003 · Mirus	MIR 6003 Mirus	0.3 ml	£191.00 0.3 ml view
TransIT-X2 Dynamic Delivery System MIR 6004 · Mirus	MIR 6004 Mirus	0.75 ml	£477.00 0.75 ml view
TransIT-X2 Dynamic Delivery System MIR 6005 · Mirus	MIR 6005 Mirus	5 x 1.5 ml	£3447.00 5 x 1.5 ml view
TransIT-X2 Dynamic Delivery System MIR 6006 · Mirus	MIR 6006 Mirus	10 x 1.5 ml	£6337.00 10 x 1.5 ml view

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CRISPR/Cas9 transfection reagent sample request