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High efficiency transfection reagents for CRISPR/Cas9

The range of high efficiency transfection reagents for CRISPR/Cas9 delivery from Mirus Bio are ideal for the efficient transfection of plasmid DNA, mRNA, gRNA and ribonucleoprotein into multiple cell types including hard-to-transfect cells. 

Transfection reagents for CRISPR/Cas9 component delivery
• TransIT-X2® - novel polymeric system ideal for delivery of CRISPR/Cas9 components in DNA, gRNA and RNP formats
• TransIT®-mRNA - high efficiency low toxicity reagent designed to deliver CRISPR/Cas9 components in mRNA and gRNA formats.
• Ingenio® - high efficiency electroporation solution compatible with most conventional electroporation devices delivering CRISPR/Cas9 components in DNA, mRNA, gRNA and RNP formats.

 
DNA
mRNA
gRNA
RNP
TransIT-X2 dynamic delivery system
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TransIT-mRNA transfection kit
 
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Ingenio electroporation solution
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DNA, RNA and protein formats for CRISPR genome editing

DNA, RNA and protein formats for CRISPR genome editing

TransIT-X2 Dynamic Delivery System was used to deliver Cas9 pDNA/gRNA and Cas9 protein/gRNA (RNP complex). TransIT-mRNA was used to deliver Cas9 mRNA/gRNA.  A T7E1 mismatch assay was used to measure cleavage efficiency at 48 hours post-transfection. For more details, see our methods for delivery of CRISPR/Cas9 DNA, RNA and RNP. 

Efficient genome editing with Cas9 plasmid DNA andguide RNA Oligonucleotides

fficient genome editing with Cas9 plasmid DNA andguide RNA Oligonucleotides

 HEK293T/17, U2OS and NHDF cells were co-transfected with 0.5 µg of Cas9 encoding pDNA (MilliporeSigma) and 50nM PPIB targeting 2-part gRNA (Dharmacon) using TransIT-X2® Dynamic Delivery System (2 µl/well of a 24-well plate, Mirus Bio).  A T7E1 mismatch detection assay was used to measure cleavage efficiency at 48 hours post-transfection. 

Material available for download
Optimisation of DNA, RNA and RNP delivery for efficient mammalian cell engineering
CRISPR brochure
Ingenio electroporation for CRISPR Cas9 RNP delivery
TransIT-mRNA for CRISPR Cas9 mRNA and gRNA delivery
TransIT-X2 for CRISPR Cas9 Plasmid and gRNA delivery
TransIT-X2 for CRISPR Cas9 Plasmid delivery
TransIT-X2 for CRISPR Cas9 RNP and Oligo delivery
TransIT-X2 for CRISPR Cas9 RNP delivery

Free samples
Request a sample of any of these transfection reagents

Amaxa is a registered trademarks of the Lonza Cologne AG in Germany and/or U.S. and/or other countries
Bio-Rad® is a registered trademark of Bio-Rad Laboratories, Inc
BTX® is a registered trademark of Harvard Apparatus

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
TransIT®-mRNA Transfection Kit MIR 2225 · Mirus
MIR 2225
Mirus
0.4 ml £482.00
0.4 ml
view
TransIT®-mRNA Transfection Kit MIR 2250 · Mirus
MIR 2250
Mirus
1 ml £860.00
1 ml
view
TransIT®-mRNA Transfection Kit MIR 2255 · Mirus
MIR 2255
Mirus
5 x 1 ml £3764.00
5 x 1 ml
view
TransIT®-mRNA Transfection Kit MIR 2256 · Mirus
MIR 2256
Mirus
10 x 1 ml £6963.00
10 x 1 ml
view
Ingenio® Electroporation Solution MIR 50111 · Mirus
MIR 50111
Mirus
25 reactions £200.00
25 reactions
view
Ingenio® Electroporation Kit for Lonza-Amaxa® Nucleofector® II/2b devices (0.2 cm cuvettes and cell droppers) MIR 50112 · Mirus
MIR 50112
Mirus
25 reactions £317.00
25 reactions
view
Ingenio® Electroporation Solution MIR 50114 · Mirus
MIR 50114
Mirus
50 reactions £317.00
50 reactions
view
Ingenio® Electroporation Kit for Lonza-Amaxa® Nucleofector® II/2b devices (0.2 cm cuvettes and cell droppers) MIR 50115 · Mirus
MIR 50115
Mirus
50 reactions £553.00
50 reactions
view
Ingenio® Electroporation Solution MIR 50117 · Mirus
MIR 50117
Mirus
100 reactions £553.00
100 reactions
view
TransIT-X2® Dynamic Delivery System MIR 6000 · Mirus
MIR 6000
Mirus
1.5 ml £727.00
1.5 ml
view
TransIT-X2® Dynamic Delivery System MIR 6003 · Mirus
MIR 6003
Mirus
0.3 ml £174.00
0.3 ml
view
TransIT-X2® Dynamic Delivery System MIR 6004 · Mirus
MIR 6004
Mirus
0.75 ml £437.00
0.75 ml
view
TransIT-X2® Dynamic Delivery System MIR 6005 · Mirus
MIR 6005
Mirus
5 x 1.5 ml £3154.00
5 x 1.5 ml
view
TransIT-X2® Dynamic Delivery System MIR 6006 · Mirus
MIR 6006
Mirus
10 x 1.5 ml £5799.00
10 x 1.5 ml
view