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ChIP-validated antibodies

Chromatin immunoprecipitation (chromatin IP or ChIP) is an extremely challenging technique which requires the use of high-quality antibodies. For an antibody to work in chromatin IP, it must be of high titre and very specific, with no detection of non-target proteins. Most importantly, it must be able to recognise the target protein in its native chromatin-associated context after fixation.

Active Motif specialises in manufacturing high-quality antibodies to histones, histone modifications and chromatin proteins, and validating them for use in ChIP, as well as ChIP-Seq and ChIP-chip. 

Benefits of using these antibodies
• High titre, highly specific antibodies
• More than 250 ChIP-validated antibodies available
• Validated for ChIP, ChIP-seq and ChIP-chip
• Antibodies to histones, histone modifications and chromatin proteins available

On this page is a small selection of antibodies available. For the full list, please download the Active Motif antibodies brochure or use our search to find your target.

Antibodies are highly validated for ChIP using multiple techniques

Histone H3 acetyl Lys27 antibody tested by ChIP qPCR analysis

Histone H3 acetyl Lys27 antibody tested by ChIP qPCR analysis. ChIP was performed using the ChIP-IT® High Sensitivity Kit with chromatin from a human hepatocyte cell line. qPCR was performed using a negative control primer set (Untr20) and three positive control primer sets that amplify known H3K27Ac sites.

Histone H3 trimethyl Lys27 pAb tested by ChIP-Seq

Histone H3 trimethyl Lys27 pAb tested by ChIP-Seq. ChIP was performed using chromatin from the human iPS 19.11 cell line. ChIP DNA was sequenced on the Illumina® GA II and sequence tags were mapped to identify H3K27me3 binding. The image shows H3K27me3 binding across a 140,000 bp region of the HOX gene cluster on chromosome 12.

5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation

5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 μl of 5-Hydroxymethylcytidine antibody (5hmC, maroon bars) or 1 μl of control rabbit IgG (IgG, blue bars). Real time quantitative PCR was performed on the immunoprecipitated DNA and results plotted as % of input DNA. The spiked APC DNA contained either no methylation (DNA), 5-methylcytosine methylation (5-mC) or 5-hydroxymethylcytosine methylation (5-hmC).

Material available for download
Active Motif antibodies for epigenetics and gene regulation brochure
Active Motif epigenetics products and services
Active Motif tools for disease research
ChIP-IT Express brochure

Illumina is a registered trademark of Illumina, Inc 

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Histone H3K27ac antibody (pAb) 39133 · Active Motif
39133
Active Motif
100 µg £469.00
100 µg
view
Histone H3ac (pan-acetyl) antibody (pAb) 39139 · Active Motif
39139
Active Motif
100 µl £469.00
100 µl
view
Histone H3K27me3 antibody (pAb) 39155 · Active Motif
39155
Active Motif
100 µg £469.00
100 µg
view
Histone H3K9me3 antibody (pAb) 39161 · Active Motif
39161
Active Motif
100 µl £469.00
100 µl
view
HIF-1 alpha antibody (pAb) 39665 · Active Motif
39665
Active Motif
100 µl £469.00
100 µl
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5-Hydroxymethylcytosine (5-hmC) antibody (pAb) 39769 · Active Motif
39769
Active Motif
100 µl £459.00
100 µl
view
Histone H3K4me3 antibody (mAb) (Clone MABI 0304) 61379 · Active Motif
61379
Active Motif
100 µg £469.00
100 µg
view
AbFlex Histone H3K9ac antibody (rAb) 91103 · Active Motif
91103
Active Motif
100 µg £384.00
100 µg
view
AbFlex EED antibody (rAb) 91135 · Active Motif
91135
Active Motif
100 µg £384.00
100 µg
view