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ChIP DNA purification: Zymo-Spin ChIP kit

The Zymo-Spin™ ChIP Kit from Zymo Research is optimised for the purification of high quality and concentrated ChIP DNA from mammalian cells. Using a microelution spin column and a simple ChIP protocol for the investigation of protein-DNA interactions, the Zymo-Spin ChIP kit eliminates the need for messy precipitations and is ideal for effectively identifying binding sites for transcription factors, co-factors and other DNA regulatory proteins.

Benefits of using the Zymo-Spin ChIP Kit
• High quality ChIP DNA produced
• Simple workflow
• No messy precipitations
• Elute in as little as 6 µl
• Mammalian cell sample optimised
• ChIP DNA is ideal for ChIP-qPCR and ChIP-Seq

Zymo-Spin ChIP Kit purification comparison

ChIP DNA Purification Comparison

 
ChIP DNA purification method Zymo Research Phenol-chloroform Supplier Q
Total time 5 min >60 min 5 min


Total and immunoprecipitated chromatin were reverse cross-linked and recovered using either the ChIP DNA Clean & Concentrator™ (included in the Zymo-Spin ChIP Kit), phenol-chloroform extraction or DNA recovery kit from Supplier Q. The amount of ChIP DNA was determined using qPCR with primers specific to the GAPDH promoter. ChIP DNA enrichment is graphed as percentage input.  

Zymo-Spin ChIP Kit recovers high-qualiy ChIP DNA

High quality ChIP DNA recovery

ENCODE Quality ChIP Workflow: Browser tracks depicting H3K4me3 ChIP-Seq assay using the Zymo-Spin ChIP Kit. Peaks overlap the same sites identified at the Broad Institute of MIT and Harvard as part of the ENCODE project.

Applications
The eluted ChIP DNA is suitable for the following applications:
• End point PCR
• Quantitative PCR
• ChIP-Seq library preparation
• Next generation sequencing

Material available for download
Zymo-Spin ChIP Kit protocol
Zymo-Spin ChIP Kit datasheet

Zymo-Spin ChIP Kit citations

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Zymo-Spin ChIP Kit D5209 · Zymo Research
D5209
Zymo Research
10 preparations £249.00
10 preparations
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Zymo-Spin ChIP Kit D5210 · Zymo Research
D5210
Zymo Research
25 preparations £507.00
25 preparations
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