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Boyden Chamber cell migration and invasion assays: CytoSelect

The CytoSelect™ assays from Cell Biolabs are an essential tool for the study of cell migration and invasion.

How CytoSelect works
The assay consists of a cylindrical cell culture insert which nests inside a well of a cell culture plate. This insert contains a polycarbonate membrane at the bottom which has a defined pore size. Cells are seeded in at the top of the insert in serum-free media, while serum of similar chemoattractants are placed in the well below. Migratory cells will move through the pores, toward the chemoattractant below and can be stained or quantified using a plate reader. Invasive cells may be similarly measured by the placement of a coating of extracellular matrix proteins on top of the membrane.

The CytoSelect Boyden Chamber assays can be used to measure cell invasion, as well as various types of cell migration:

Assay type Definition Cell types Pore size Insert coating Catalogue number(s)
Chemotaxis Migration of cells toward a chemoattractant (chemical signal) in a cell's surrounding environment Leukocytes
Neutrophils
3 µm None CBA-103
CBA-104
Lymphocytes
Macrophages
Monocyte
5 µm None CBA-102
CBA-105
Endothelial cells
Epithelial cells
Fibroblasts
Tumour cells
8 µm None CBA-100
CBA-106
Astrocytes
Slow-moving cells
12 µm None CBA-107
Haptotaxis Migration of cells along a gradient of cellular adhesion sites or extracellular matrix-bound chemoattractants Endothelial cells
Epithelial cells
Fibroblasts
8 µm Collagen I (bottom) CBA-100-COL
Fibronectin (bottom) CBA-100-FN
Transmigration Migration of cells through the vascular endothelium toward a chemoattractant Leukocytes 3 µm None CBA-212
Tumour Cells 8 µm None CBA-216
Invasion Movement of cells through the 3D extracellular matrix into neighbouring tissues; includes ECM degradation and proteolysis Endothelial cells
Epithelial cells
Fibroblasts
Tumour cells
8 µm ECM Matrix (top) CBA-110
CBA-112
Collagen I (top) CBA-111-COL
CBA-112-COL
Laminin I (top) CBA-110-LN
CBA-112-LN

 

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
CytoSelect™ 24-well Cell Migration Assay (3 µm), Fluorometric CBA-103 · Cell Biolabs
CBA-103
Cell Biolabs
12 assays £595.00
12 assays
view
CytoSelect™ 24-well Cell Migration Assay (8 µm), Colorimetric CBA-100 · Cell Biolabs
CBA-100
Cell Biolabs
12 assays £582.00
12 assays
view
CytoSelect™ 24-well Cell Haptotaxis Assay (8 µm), COL-coated, Colorimetric CBA-100-COL · Cell Biolabs
CBA-100-COL
Cell Biolabs
12 assays £621.00
12 assays
view
CytoSelect™ 24-well Cell Haptotaxis Assay (8 µm), FN-coated, Colorimetric CBA-100-FN · Cell Biolabs
CBA-100-FN
Cell Biolabs
12 assays £621.00
12 assays
view
CytoSelect™ 24-well Cell Migration Assay (5 µm), Fluorometric CBA-102 · Cell Biolabs
CBA-102
Cell Biolabs
12 assays £595.00
12 assays
view
CytoSelect™ 96-well Cell Migration Assay (3 µm), Fluorometric CBA-104 · Cell Biolabs
CBA-104
Cell Biolabs
96 assays £699.00
96 assays
view
CytoSelect™ 96-well Cell Migration Assay (5 µm), Fluorometric CBA-105 · Cell Biolabs
CBA-105
Cell Biolabs
96 assays £699.00
96 assays
view
CytoSelect™ 96-well Cell Migration Assay (8 µm), Fluorometric CBA-106 · Cell Biolabs
CBA-106
Cell Biolabs
96 assays £699.00
96 assays
view
CytoSelect™ 24-well Cell Migration Assay (12 µm), Colorimetric CBA-107 · Cell Biolabs
CBA-107
Cell Biolabs
12 assays £595.00
12 assays
view
CytoSelect™ 24-well Cell Invasion Assay, Colorimetric CBA-110 · Cell Biolabs
CBA-110
Cell Biolabs
12 assays £647.00
12 assays
view
CytoSelect™ 24-well Laminin Cell Invasion, Colorimetric CBA-110-LN · Cell Biolabs
CBA-110-LN
Cell Biolabs
12 assays £647.00
12 assays
view
CytoSelect™ 24-well Collagen Cell Invasion Assay, Fluorometric CBA-111-COL · Cell Biolabs
CBA-111-COL
Cell Biolabs
12 assays £647.00
12 assays
view
CytoSelect™ 96-well Cell Invasion, Fluorometric CBA-112 · Cell Biolabs
CBA-112
Cell Biolabs
96 assays £849.00
96 assays
view
CytoSelect™ 96-well Collagen Cell Invasion Assay, Fluorometric CBA-112-COL · Cell Biolabs
CBA-112-COL
Cell Biolabs
96 assays £849.00
96 assays
view
CytoSelect™ 96-well Laminin Cell Invasion Assay, Fluorometric CBA-112-LN · Cell Biolabs
CBA-112-LN
Cell Biolabs
96 assays £849.00
96 assays
view
CytoSelect™ Leukocyte Transmigration Assay CBA-212 · Cell Biolabs
CBA-212
Cell Biolabs
24 assays £817.00
24 assays
view
CytoSelect™ Tumor Transendothelial Migration Assay CBA-216 · Cell Biolabs
CBA-216
Cell Biolabs
24 assays £817.00
24 assays
view