Boyden Chamber cell migration and invasion assays: CytoSelect

The CytoSelect™ assays from Cell Biolabs are an essential tool for the study of cell migration and invasion.

How CytoSelect works
The assay consists of a cylindrical cell culture insert which nests inside a well of a cell culture plate. This insert contains a polycarbonate membrane at the bottom which has a defined pore size. Cells are seeded in at the top of the insert in serum-free media, while serum of similar chemoattractants are placed in the well below. Migratory cells will move through the pores, toward the chemoattractant below and can be stained or quantified using a plate reader. Invasive cells may be similarly measured by the placement of a coating of extracellular matrix proteins on top of the membrane.

The CytoSelect Boyden Chamber assays can be used to measure cell invasion, as well as various types of cell migration:

Assay type	Definition	Cell types	Pore size	Insert coating	Catalogue number(s)
Chemotaxis	Migration of cells toward a chemoattractant (chemical signal) in a cell's surrounding environment	Leukocytes Neutrophils	3 µm	None	CBA-103 CBA-104
		Lymphocytes Macrophages Monocyte	5 µm	None	CBA-102 CBA-105
		Endothelial cells Epithelial cells Fibroblasts Tumour cells	8 µm	None	CBA-100 CBA-106
		Astrocytes Slow-moving cells	12 µm	None	CBA-107
Haptotaxis	Migration of cells along a gradient of cellular adhesion sites or extracellular matrix-bound chemoattractants	Endothelial cells Epithelial cells Fibroblasts	8 µm	Collagen I (bottom)	CBA-100-COL
Haptotaxis		Endothelial cells Epithelial cells Fibroblasts	8 µm	Fibronectin (bottom)	CBA-100-FN
Transmigration	Migration of cells through the vascular endothelium toward a chemoattractant	Leukocytes	3 µm	None	CBA-212
Transmigration		Tumour Cells	8 µm	None	CBA-216
Invasion	Movement of cells through the 3D extracellular matrix into neighbouring tissues; includes ECM degradation and proteolysis	Endothelial cells Epithelial cells Fibroblasts Tumour cells	8 µm	ECM Matrix (top)	CBA-110 CBA-112
				Collagen I (top)	CBA-111-COL CBA-112-COL
				Laminin I (top)	CBA-110-LN CBA-112-LN

Products

Note: product availability depends on country - see product detail page.

Details	Cat number & supplier	Size	Price
CytoSelect™ 24-well Cell Migration Assay (3 µm), Fluorometric CBA-103 · Cell Biolabs	CBA-103 Cell Biolabs	12 assays	£572.00 12 assays view
CytoSelect™ 24-well Cell Migration Assay (8 µm), Colorimetric CBA-100 · Cell Biolabs	CBA-100 Cell Biolabs	12 assays	£572.00 12 assays view
CytoSelect™ 24-well Cell Haptotaxis Assay (8 µm), COL-coated, Colorimetric CBA-100-COL · Cell Biolabs	CBA-100-COL Cell Biolabs	12 assays	£610.00 12 assays view
CytoSelect™ 24-well Cell Haptotaxis Assay (8 µm), FN-coated, Colorimetric CBA-100-FN · Cell Biolabs	CBA-100-FN Cell Biolabs	12 assays	£610.00 12 assays view
CytoSelect™ 24-well Cell Migration Assay (5 µm), Fluorometric CBA-102 · Cell Biolabs	CBA-102 Cell Biolabs	12 assays	£572.00 12 assays view
CytoSelect™ 96-well Cell Migration Assay (3 µm), Fluorometric CBA-104 · Cell Biolabs	CBA-104 Cell Biolabs	96 assays	£691.00 96 assays view
CytoSelect™ 96-well Cell Migration Assay (5 µm), Fluorometric CBA-105 · Cell Biolabs	CBA-105 Cell Biolabs	96 assays	£691.00 96 assays view
CytoSelect™ 96-well Cell Migration Assay (8 µm), Fluorometric CBA-106 · Cell Biolabs	CBA-106 Cell Biolabs	96 assays	£691.00 96 assays view
CytoSelect™ 24-well Cell Migration Assay (12 µm), Colorimetric CBA-107 · Cell Biolabs	CBA-107 Cell Biolabs	12 assays	£572.00 12 assays view
CytoSelect™ 24-well Cell Invasion Assay (Basement Membrane), Colorimetric CBA-110 · Cell Biolabs	CBA-110 Cell Biolabs	12 assays	£635.00 12 assays view
CytoSelect™ 24-well Collagen Cell Invasion Assay, Fluorometric CBA-111-COL · Cell Biolabs	CBA-111-COL Cell Biolabs	12 assays	£635.00 12 assays view
CytoSelect™ 96-well Cell Invasion Assay (Basement Membrane), Fluorometric CBA-112 · Cell Biolabs	CBA-112 Cell Biolabs	96 assays	£836.00 96 assays view
CytoSelect™ 96-well Collagen Cell Invasion Assay, Fluorometric CBA-112-COL · Cell Biolabs	CBA-112-COL Cell Biolabs	96 assays	£836.00 96 assays view
CytoSelect™ Leukocyte Transmigration Assay CBA-212 · Cell Biolabs	CBA-212 Cell Biolabs	24 assays	£798.00 24 assays view
CytoSelect™ Tumor Transendothelial Migration Assay CBA-216 · Cell Biolabs	CBA-216 Cell Biolabs	24 assays	£798.00 24 assays view

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