Bioluminescence and fluorescence imaging vectors

These bioluminescence imaging (BLI) vectors, fluorescence imaging (FLI) vectors and minicircle DNA constructs and D-Luciferin reagents perform molecular imaging in vivo.

Dual bioluminescent and fluorescent vectors
We offer BLI and FLI dual reporter lentivectors and minicircle DNA constructs based on System Bioscience's unique vector technology for in vivo and in vitro imaging. These reporter vectors utilise either GFP or RFP fluorescence with a fire fly luciferase reporter.

Lentiviral vectors and prepackaged virus
For high efficiency gene delivery, the BLI lentivectors can be packaged into pseudoviruses and used to infect most cell types both in vitro and in vivo. As viruses, lenti-based vectors have nearly 100 percent integration so they are suitable for establishing stable cell lines and for use in high-throughput applications. In addition lenti-based BLIV vectors can be used as regular plasmid DNA with transient transfection protocols.

Minicircle vectors
Minicircle DNA BLI vector technology provides an innovative means to effectively deliver and express the reporters as nonintegrating episomes. These minicircle DNAs express the BLI markers in cells and tissues for much longer and at much higher levels as compared to regular plasmid DNA.

In vitro applications
BLI lentiviral vectors can be used to generate stable cell lines or Minicircle vectors used to make nonviral and long-lasting (several weeks) reporter cells.

In vivo applications
Bioluminescent and fluorescent cell lines can be injected or the vectors directly used in animal models through hydrodynamic tail vein injections.

Parental minicircle plasmids are available for purchase by not-for-profit researchers only. Commercial users may purchase pre-made mcDNA [ready-to-transfect] only. For any other purposes, including the ability to buy the parental MC production system, commercial users should contact Cambridge Bioscience for further information.

Material available for download
Bioluminescent and Fluorescent Imaging Vectors user manual
 

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Minicircle Dual Reporter: EF1-GFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV503MN-1 · System Biosciences
BLIV503MN-1
System Biosciences
10 µg £821.00
10 µg
view
Minicircle Dual Reporter: UBC-RFP-T2A-Luciferase DNA (30 ug) BLIV504MC-1 · System Biosciences
BLIV504MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: UBC-RFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV504MN-1 · System Biosciences
BLIV504MN-1
System Biosciences
10 µg £821.00
10 µg
view
Lentiviral Dual Reporter: CMV-GFP-T2A-Luciferase plasmid (10 ug) BLIV101PA-1 · System Biosciences
BLIV101PA-1
System Biosciences
10 µg £671.00
10 µg
view
Lentiviral Dual Reporter: CMV-GFP-T2A-Luciferase pre-packaged virus BLIV101VA-1 · System Biosciences
BLIV101VA-1
System Biosciences
>2 million IFU £671.00
>2 million IFU
view
Minicircle Dual Reporter: UBC-GFP-T2A-Luciferase DNA (30 ug) BLIV505MC-1 · System Biosciences
BLIV505MC-1
System Biosciences
30 µg £594.00
30 µg
view
Lentiviral Triple Reporter: CMV-Luciferase-RFP-TK plasmid (10 ug) BLIV102PA-1 · System Biosciences
BLIV102PA-1
System Biosciences
10 µg £671.00
10 µg
view
Minicircle Dual Reporter: UBC-GFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV505MN-1 · System Biosciences
BLIV505MN-1
System Biosciences
10 µg £821.00
10 µg
view
Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase DNA (30 ug) BLIV506MC-1 · System Biosciences
BLIV506MC-1
System Biosciences
30 µg £594.00
30 µg
view
Lentiviral Triple Reporter: CMV-Luciferase-RFP-TK pre-packaged virus BLIV102VA-1 · System Biosciences
BLIV102VA-1
System Biosciences
>2 million IFU £671.00
>2 million IFU
view
Lentiviral Dual Reporter: UBC-RFP-T2A-Luciferase plasmid (10 ug) BLIV200PA-1 · System Biosciences
BLIV200PA-1
System Biosciences
10 µg £671.00
10 µg
view
Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV506MN-1 · System Biosciences
BLIV506MN-1
System Biosciences
10 µg £821.00
10 µg
view
Lentiviral Dual Reporter: UBC-RFP-T2A-Luciferase pre-packaged virus BLIV200VA-1 · System Biosciences
BLIV200VA-1
System Biosciences
>2 million IFU £671.00
>2 million IFU
view
Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase DNA (30 ug) BLIV507MC-1 · System Biosciences
BLIV507MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV507MN-1 · System Biosciences
BLIV507MN-1
System Biosciences
10 µg £821.00
10 µg
view
Minicircle Single Reporter: CMV-Luciferase Minicircle (30 ug) BLIV510MC-1 · System Biosciences
BLIV510MC-1
System Biosciences
30 µg £594.00
30 µg
view
Lentiviral Triple Reporter: UBC-Luciferase-RFP-TK plasmid (10 ug) BLIV202PA-1 · System Biosciences
BLIV202PA-1
System Biosciences
10 µg £671.00
10 µg
view
Minicircle Single Reporter: CMV-Luciferase Parental Plasmid (10 ug) BLIV510MN-1 · System Biosciences
BLIV510MN-1
System Biosciences
10 µg £821.00
10 µg
view
Lentiviral Triple Reporter: UBC-Luciferase-RFP-TK pre-packaged virus BLIV202VA-1 · System Biosciences
BLIV202VA-1
System Biosciences
>2 million IFU £671.00
>2 million IFU
view
Lentiviral Triple Reporter: MSCV-Luciferase-RFP-TK plasmid (10 ug) BLIV302PA-1 · System Biosciences
BLIV302PA-1
System Biosciences
10 µg £671.00
10 µg
view
Lentiviral Triple Reporter: MSCV-Luciferase-RFP-TK pre-packaged virus BLIV302VA-1 · System Biosciences
BLIV302VA-1
System Biosciences
>2 million IFU £671.00
>2 million IFU
view
Minicircle Dual Reporter: CMV-RFP-T2A-Luciferase DNA (30 ug) BLIV500MC-1 · System Biosciences
BLIV500MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: CMV-RFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV500MN-1 · System Biosciences
BLIV500MN-1
System Biosciences
10 µg £821.00
10 µg
view
Minicircle Dual Reporter: CMV-GFP-T2A-Luciferase DNA (30 ug) BLIV501MC-1 · System Biosciences
BLIV501MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: CMV-GFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV501MN-1 · System Biosciences
BLIV501MN-1
System Biosciences
10 µg £821.00
10 µg
view