We offer a range of electrocompetent cells from Lucigen ideal for BAC cloning applications.
Efficiency | Applications | |
---|---|---|
BigEasy® TSA™ electrocompetent cells |
> 2 × 1010 cfu/µg pKanR DNA |
For use with Lucigen’s pJAZZ®-OC or OK linear vectors in the BigEasy v2.0 linear cloning system. Containing the telN gene required for high efficiency transformation and the antA gene required for copy number control with the pJAZZ vector integrated into the host genome, these cells offer high yields and high-quality plasmid DNA. |
BAC-Optimised Replicator™ v2.0 Electrocompetent Cells |
> 1 × 1010 cfu/µg pKanR DNA |
Required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. |
E. cloni® 10G BAC-Optimised Electrocompetent Cells |
> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA |
For large DNA cloning. Manufactured using a novel procedure that maximises transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation. |
Benefits of using these cells
• Highest available transformation efficiencies for library construction and screening
• Optimised for high yields of DNA
Material available for download
BigEasy-TSA Electrocompetent Cells Manual
E. cloni 10G BAC-Optimised Electrocompetent cells protocol
BAC-Optimised Replicator v2.0 Electrocompetent Cells protocol
Products
Note: product availability depends on country - see product detail page.
Details | Cat number & supplier | Size | Price |
BAC-Optimized Replicator v2.0 Electrocompetent Cells 60210-1 · Lucigen | 60210-1 Lucigen |
12 reactions duos |
£309.00
12 reactions duos
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BAC-Optimized Replicator v2.0 Electrocompetent Cells 60210-2 · Lucigen | 60210-2 Lucigen |
24 reactions duos |
£532.00
24 reactions duos
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E. cloni 10G BAC-Optimized Electrocompetent Cells 60215-2 · Lucigen | 60215-2 Lucigen |
25 reactions fivers |
£507.00
25 reactions fivers
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