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BAC cloning competent cells

We offer a range of electrocompetent cells from Lucigen ideal for BAC cloning applications.

  Efficiency Applications

BAC-Optimised Replicator™ v2.0 Electrocompetent Cells

> 1 × 1010 cfu/µg pKanR DNA

Required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields.
E. cloni® 10G BAC-Optimised Electrocompetent Cells

> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA

For large DNA cloning.

Manufactured using a novel procedure that maximises transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.


Benefits of using these cells
• Highest available transformation efficiencies for library construction and screening
• Optimised for high yields of DNA

Material available for download
E. cloni 10G BAC-Optimised Electrocompetent cells protocol
BAC-Optimised Replicator v2.0 Electrocompetent Cells protocol

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
BAC-Optimized Replicator v2.0 Electrocompetent Cells, 12 Reactions (DUOs) 60210-1 · Lucigen
60210-1
Lucigen
1 each £381.00
1 each
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BAC-Optimized Replicator v2.0 Electrocompetent Cells, 24 Reactions (DUOs) 60210-2 · Lucigen
60210-2
Lucigen
1 each £654.00
1 each
view
E. cloni 10G BAC-Optimized Electrocompetent Cells, 25 rxns (Fivers) 60215-2 · Lucigen
60215-2
Lucigen
1 each £625.00
1 each
view