- In this section:
- Request an E. cloni 10G ELITE Electrocompetent Cells sample
- Request an E. cloni 10G chemically competent cells sample
- E. cloni 10G chemically competent cells manual
- E. cloni 96-well chemically competent cells manual
- E. cloni 5-alpha chemically competent cells manual
- E. cloni 10G and 10GF´ electrocompetent cells manual
- E. cloni competent cells summary
- E. cloni citations
The E. cloni® competent cells from Lucigen incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability. Available in chemically and electrocompetent formats in a range of high transformation efficiencies, the E.cloni cells are ideal as direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10 and XL1-Blue).
Benefits of the E. cloni range
• High yield optimised
• Directly replace standard cloning strains
• phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable
• Range of high electro competent transformation efficiencies (5 x 109 to 4 x 1010 cfu/µg)
• Range of high chemically competent transformation efficiencies (1 x 106 to 1 x 109 cfu/µg)
| Format | Name | Efficiency | Applications |
|---|---|---|---|
| Electrocompetent | E. cloni 10G SUPREME Electrocompetent Cells | > 4 x 1010 cfu/µg pUC DNA | SUPREME Cells are suitable for construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible. |
| E. cloni 10G & 10GF´ ELITE Electrocompetent Cells | > 2 x 1010 cfu/µg pUC DNA | ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA. | |
| E. cloni 10G CLASSIC Electrocompetent Cells | > 5 x 109 cfu/µg pUC DNA | These cells are the most economical choice for standard cloning and library construction. | |
| Chemically competent | E. cloni 10G Chemically Competent Cells | > 1 x 109 cfu/µg pUC DNA | Highly efficient competent cells for routine cloning applications. Available in 96-well well format for higher throughput. |
| E. cloni 10G Chemically Competent Cells, Subcloning Grade | > 1 x 106 cfu/µg pUC DNA | The best value for simple cloning and plasmid propagation. | |
| E. cloni 5-alpha Chemically Competent Cells | > 1 x 108 cfu/µg pUC DNA | Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening. |
Material available for download
E. cloni competent cells summary
E. cloni 10G and 10GF´ electrocompetent cells manual
E. cloni 10G chemically competent cells manual
E. cloni 5-alpha chemically competent cells manual
E. cloni 96-well chemically competent cells manual
DH5α and DH10B are trademarks of Life Technologies Inc
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| E. cloni 10G Supreme Electrocompetent Cells, 12 Reactions (SOLOs) 60081-1 · LGC Lucigen | 60081-1 LGC Lucigen |
12 reactions (solos) |
£583.00
12 reactions (solos)
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| E. cloni 10G Supreme Electrocompetent Cells, 12 Reactions (DUOs) 60080-1 · LGC Lucigen | 60080-1 LGC Lucigen |
12 reactions (duos) |
£513.00
12 reactions (duos)
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| E. cloni 10G Supreme Electrocompetent Cells, 24 Reactions (DUOs) 60080-2 · LGC Lucigen | 60080-2 LGC Lucigen |
24 reactions (duos) |
£938.00
24 reactions (duos)
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| E. cloni 10GF' Electrocompetent Cells, 12 Rxns (DUOs) 60061-1 · LGC Lucigen | 60061-1 LGC Lucigen |
12 reactions (duos) |
£337.00
12 reactions (duos)
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| E. cloni 10GF' Electrocompetent Cells, 24 Reactions (DUOs) 60061-2 · LGC Lucigen | 60061-2 LGC Lucigen |
24 reactions (duos) |
£575.00
24 reactions (duos)
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| E. cloni 10G Elite Electrocompetent Cells, 12 Reactions (DUOs) 60052-1 · LGC Lucigen | 60052-1 LGC Lucigen |
12 reactions (duos) |
£331.00
12 reactions (duos)
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| E. cloni 10G Elite Electrocompetent Cells, 24 Reactions (DUOs) 60052-2 · LGC Lucigen | 60052-2 LGC Lucigen |
24 reactions (duos) |
£566.00
24 reactions (duos)
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| E. cloni 10G Elite Electrocompetent Cells, 24 Reactions (SixPacks) 60052-3 · LGC Lucigen | 60052-3 LGC Lucigen |
24 reactions (sixpacks) |
£438.00
24 reactions (sixpacks)
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| E. cloni 10G Elite Electrocompetent Cells, 48 Reactions (SixPacks) 60052-4 · LGC Lucigen | 60052-4 LGC Lucigen |
48 reactions (sixpacks) |
£721.00
48 reactions (sixpacks)
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| E. cloni 10G Classic Electrocompetent Cells, 24 Reactions (SixPacks) 60117-1 · LGC Lucigen | 60117-1 LGC Lucigen |
24 reactions (sixpacks) |
£389.00
24 reactions (sixpacks)
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| E. cloni 10G Classic Electrocompetent Cells, 48 Reactions (SixPacks) 60117-2 · LGC Lucigen | 60117-2 LGC Lucigen |
48 reactions (sixpacks) |
£693.00
48 reactions (sixpacks)
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| E. cloni 10G Chemically Competent Cells, 12 Rxns (DUOs) 60107-1 · LGC Lucigen | 60107-1 LGC Lucigen |
12 reactions (duos) |
£244.00
12 reactions (duos)
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| E. cloni 10G Chemically Competent Cells, 24 Reactions (DUOs) 60107-2 · LGC Lucigen | 60107-2 LGC Lucigen |
24 reactions (duos) |
£427.00
24 reactions (duos)
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| E. cloni 10G Chemically Competent Cells, Subcloning Grade, 48 Reactions 60108-1 · LGC Lucigen | 60108-1 LGC Lucigen |
Subcloning grade |
£143.00
Subcloning grade
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| E. cloni 5-alpha Chemically Competent Cells, 12 Reactions (DUOs) 60602-1 · LGC Lucigen | 60602-1 LGC Lucigen |
12 reactions (duos) |
£221.00
12 reactions (duos)
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In this section
Request an E. cloni 10G ELITE Electrocompetent Cells sample- Request an E. cloni 10G chemically competent cells sample
- E. cloni 10G chemically competent cells manual
- E. cloni 96-well chemically competent cells manual
- E. cloni 5-alpha chemically competent cells manual
- E. cloni 10G and 10GF´ electrocompetent cells manual
- E. cloni competent cells summary
- E. cloni citations