Blocking Buffer Sampler Kit

Name: Blocking Buffer Sampler Kit
Brand: Rockland Immunochemicals
SKU: CBSKU_100825819
Price: 261 GBP
Availability: InStock

Catalogue number:

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K505

Supplier:

Rockland Immunochemicals

Size:

1 kit _$$_

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£261.00

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Estimated delivery Thu 9 May
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Alternative Names:

Blocking Buffer Sampler Kit, Normal Goat Serum, NGS, BSA, bovine serum albumin, blocking buffer for fluorescent western blotting, ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free), TBS Fish Gel Solution

Product Description:

Rockland lyophilized NORMAL GOAT SERUM (NGS) is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications, BOVINE SERUM ALBUMIN is suitable dilute solutions of antibody, use as a blocking agent to reduce non-specific binding, 2X Blocking Buffer for Fluorescent Western Blotting is optimized for Fluorescent Western blotting and other immunoassays. ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) , 10X TBS Fish Gel Concentrate (Azide and Mercury free) . Blocking Buffer Sampler Kit contains: NORMAL GOAT SERUM (NGS) , BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free) , Blocking Buffer (2X) for Fluorescent Western Blotting, ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) - Designed for ELISA assays, 10X TBS Fish Gel Concentrate (Azide and Mercury free) is a blocking agent for immunoassays and western blot.

Storage Temperature:

Do not freeze kit. Store kit at 4°C. Kit contains multiple components. See kit insert for complete i

Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications.

This blocking buffer is specifically designed for western blotting using fluorochrome conjugated antibodies and can be used for membrane blocking and to dilute both primary and secondary antibodies. See www.rockland-inc.com for specific protocols. This buffer was prepared using ultra-pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an antimicrobial agent.

Rockland Immunochemicals produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabalizer (p/n MB-063-0100) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n MB-064-1000) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n MB-066-0100), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer (p/n MB-060-0100), added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n TMBE-1000) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.

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