Human Progranulin ELISA Kit

Granulin, also known as Proepithelin (PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 100ng/ml, 50ng/ml, 25ng/ml, 1.25ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml human Progranulin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well) . Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) , saliva, urine or human milk to each empty well. We recommend that each human Progranulin standard solution and each sample is measured in duplicate.