www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Mouse IgG2a Antibody ATTO 550 Conjugated Pre-adsorbed

Catalogue number:
610-154-041
Size:
500 µg _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£404.00 Estimated delivery Thu 23 May
Shipping is calculated in checkout
Applications:

FLISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot

Antibody Host:

Goat

Species Reactivity:

Mus musculus (Mouse)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Mouse IgG2a heavy chain

Alternative Names:

Goat anti-mouse IgG2a antibody ATTO550 conjugation, goat anti-mouse IgG2a (gamma 2a) ATTO 550 conjugated antibody

image 0
image 1
Product Description:

Anti-Mouse IgG2a ATTO 550 Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain) . Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25% of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Mouse IgG2a secondary antibody is available in a variety of formats. Anti-Mouse IgG2a ATTO 550 Antibody has been tested by dot blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Anti-Mouse IgG2a secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG2a antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Storage Temperature:

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or

ATTO ® dyes can be used for multicolor i
ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH
Atto™ dyes can be used for multicolor im
Atto™ dyes can be used for multicolor immunofluorescent detection with low background and high signal. Example shown here is Immunohistochemical staining using ATTO-550 Anti-Aquaporin 2-antibody (red) of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Nuclei are visualized with Hoechst 33342 (blue). Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH
Details Cat number & supplier Size Price
Donkey anti-Goat IgG Heavy and Light Chain Antibody HRP Conjugated A50-101P · Bethyl Laboratories, Inc.
A50-101P
Bethyl Laboratories, Inc.
1 mg £114.00
1 mg
view