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Mouse IgG (H&L) Antibody Rhodamine Conjugated

Catalogue number:
610-1002-0500
Size:
500 µg _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£82.00 Estimated delivery Thu 9 May
Shipping is calculated in checkout
Applications:

FLISA, Flow Cytometry, IF Microscopy

Antibody Host:

Goat

Species Reactivity:

Mus musculus (Mouse)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Mouse IgG whole molecule

Alternative Names:

goat anti-Mouse IgG rhodamine conjugated Antibody, goat anti-Mouse IgG Antibody TRITC conjugation

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Product Description:

Anti-Mouse IgG Rhodamine Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them) , activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F (c) region, recognized by high-affinity Fc receptor proteins, as well as the F (ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG Rhodamine Antibody has been tested by dot blot and western blot and is available in a variety of formats. Anti IgG secondary antibody conjugated is suitable for ELISA, Immunohistochemistry western blotting as well as other peroxidase antibody based assays.

Storage Temperature:

Store vial at 4°C prior to restoration.   For extended storage aliquot contents and freeze at -20°C

Western Blot of Rhodamine Conjugated Goa
Western Blot of Rhodamine Conjugated Goat anti-Mouse IgG Secondary Antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.
Dot Blot of Rhodamine Conjugated Goat-a-
Dot Blot of Rhodamine Conjugated Goat-a-Mouse IgG. Antigen: Mouse IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng Primary antibody: none Secondary antibody: Rhodamine Conjugated Goat-a-Mouse IgG secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.
Details Cat number & supplier Size Price
Donkey anti-Goat IgG Heavy and Light Chain Antibody HRP Conjugated A50-101P · Bethyl Laboratories, Inc.
A50-101P
Bethyl Laboratories, Inc.
1 mg £114.00
1 mg
view