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Rabbit anti-MED1 Recombinant Monoclonal Antibody [BLR037F]

Catalogue number:
A700-037-T
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
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£95.00 Estimated delivery Wed 28 Apr
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Recombinant antibody was purified from cell culture supernatant.

Product Type:

Antibodies

Alternative Names:

activator-recruited cofactor 205 kDa component, ARC205, CRSP1, CRSP200, DRIP205, DRIP230, Mediator complex subunit 1, mediator of RNA polymerase II transcription subunit 1, p53 regulatory protein RB18A, PBP, peroxisome proliferator-activated receptor-bind

Species Reactivity:

Human, Mouse

Immunogen:

between 1525 and 1581 (C-term)

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Formulation:

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide

Antibody Type:

Recombinant Monoclonal

UNSPSC Code:

12352203

Detection of human MED1 by western blot.

Detection of human MED1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody [BLR037F] (A700-037-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds.

Detection of human MED1 by western blot

Detection of human MED1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MED1 recombinant monoclonal antibody [BLR037F] (A700-037-T lot 1) used for IP at 20 µl per reaction. MED1 was also immunoprecipitated by rabbit anti-MED1 antibody (A300-793A). For blotting immunoprecipitated MED1, A700-037-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.

Detection of human MED1 by immunohistoch

Detection of human MED1 by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody [BLR037F] (A700-037-T lot 1) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Detection of mouse MED1 by immunohistoch

Detection of mouse MED1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody [BLR037F] (A700-037-T lot 1) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Detection of human MED1 by immunocytoche

Detection of human MED1 by immunocytochemistry. Sample: FFPE section of human HCT 116 cells. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody [BLR037F] (A700-037-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.