www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Goat anti-Rad17 Antibody, Affinity Purified

Catalogue number:
A305-788A-M
Size:
100 µl (10 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£227.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to Rad17 immobilized on solid support. The epitope recognized by A305-788A-M maps to a region between residue 564 and 614 of human RAD17 checkpoint clamp loader component using the numbering given in entry O75943 (GeneID 5884). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Product Type:

Antibodies

Alternative Names:

CCYC, cell cycle checkpoint protein (RAD17), cell cycle checkpoint protein RAD17, HRAD17, R24L, RAD1 homolog, RAD17 homolog, Rad17-like protein, RAD17SP, RAD24, RF-C activator 1 homolog, RF-C/activator 1 homolog

Species Reactivity:

Human

Immunogen:

between 564 and 614

Storage Temp:

2 - 8°C

Antibody Host:

Goat

Antibody Isotype:

Whole IgG

Formulation:

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Rad17 by western blot

Detection of human Rad17 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad17 antibody A305-788A-M (lot A305-788A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Rad17 by western blot

Detection of human Rad17 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rad17 antibody A305-788A-M (lot A305-788A-M-1) used for IP at 40 µl per reaction. Rad17 was also immunoprecipitated by rabbit anti-Rad17 antibody BL239. For blotting immunoprecipitated Rad17, A305-788A-M was used at 1:100. Detection: Chemiluminescence with an exposure time of 30 seconds.