AA408787, E3 SUMO-protein ligase TRIM28, KAP-1, KRAB-A-interacting protein, KRIP-1, MommeD9, RING-type E3 ubiquitin transferase TIF1-beta, Tif1b, Tif1beta, TIF1-beta, transcription intermediary factor 1-beta, transcriptional intermediary factor 1, beta, t
surrounding serine 824
2 - 8°C
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 cells treated with etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody A304-146A-M (lot A304-146A-M-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total KAP-1 using affinity purified rabbit anti-KAP-1 antibody A300-145A at 0.4 µg/ml with an exposure time of 3 seconds.
Detection of mouse Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from TCMK-1 cells treated with etoposide (+) and/or Lambda PP (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody (A304-145A) used for IP at 6 µg per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), A304-146A-M was used at 1:250. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-KAP-1 antibody A304-145A.