Antibody was affinity purified using an epitope specific to MMS21 immobilized on solid support. The epitope recognized by A304-126A maps to a region between residue 1 to 50 of human Non-SMC Element 2 using the numbering given in entry NP_775956.1 (GeneID 286053). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C8orf36, E3 SUMO-protein ligase NSE2, E3 SUMO-protein transferase NSE2, hMMS21, methyl methanesulfonate sensitivity gene 21, MMS21, MMS21 homolog, Non-SMC element 2 homolog, non-SMC element 2, MMS21 homolog, non-structural maintenance of chromosomes eleme
Between 1 and 50
2 - 8°C
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Detection of human MMS21 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MMS21 antibody A304-126A-M (lot A304-126A-M-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.