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Rabbit anti-IRF1 Recombinant Monoclonal Antibody [BLR039F]

Catalogue number:
A700-039-T
Size:
10 µl (5+ tests)
Product is available in:
  • UK
  • Ireland
  • Europe
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Recombinant antibody was purified from cell culture supernatant.

Product Type:

Antibodies

Alternative Names:

interferon regulatory factor 1, interferon regulatory factor 1 isoform +I9, interferon regulatory factor 1 isoform d78, interferon regulatory factor 1 isoform d9,10+, interferon regulatory factor 1 isoform delta4, interferon regulatory factor 1 isoform de

Species Reactivity:

Human

Immunogen:

between 250 and 300

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Formulation:

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide

Antibody Type:

Recombinant Monoclonal

UNSPSC Code:

12352203

Detection of human IRF1 by immunohistoch

Detection of human IRF1 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-IRF1 recombinant monoclonal antibody [BLR039F] (A700-039-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Detection of human IRF1 by immunocytoche

Detection of human IRF1 by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Rabbit anti-IRF1 recombinant monoclonal antibody [BLR039F] (A700-039-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Detection of human IRF1 by western blot.

Detection of human IRF1 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, Hep-G2, HeLa (IFNgamma treated), HeLa, SR, MOLT-4, RPMI-8226, KG-1, A-549, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-IRF1 recombinant monoclonal antibody [BLR039F] (A700-039A-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH (A300-639A).

Detection of human IRF1 by western blot

Detection of human IRF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-IRF1 recombinant monoclonal antibody [BLR039F] (A700-039A-T lot 1) used for IP at 20 µl per reaction. IRF1 was also immunoprecipitated by rabbit anti-IRF1 recombinant monoclonal antibody [BL428-1A9]. For blotting immunoprecipitated IRF1, A700-039A-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.