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Rabbit anti-VISTA Recombinant Monoclonal Antibody [BLR035F]

Catalogue number:
A700-035-T
Size:
10 µl (5+ tests)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
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Recombinant antibody was purified from cell culture supernatant.

Product Type:

Antibodies

Alternative Names:

B7H5, B7-H5, C10orf54, DD1alpha, Death Domain1alpha, GI24, PD-1H, PDCD1 homolog, platelet receptor GI24, PP2135, SISP1, sisp-1, stress-induced secreted protein-1, V-domain Ig suppressor of T cell activation, VISTA, V-set domain-containing immunoregulatory

Species Reactivity:

Human

Immunogen:

between 250 and 311 (C-term)

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Formulation:

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide

Antibody Type:

Recombinant Monoclonal

UNSPSC Code:

12352203

Detection of human VISTA by immunocytoch

Detection of human VISTA by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (A700-035-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Detection of human VISTA by immunohistoc

Detection of human VISTA by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (A700-035-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Detection of human VISTA by western blot

Detection of human VISTA by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, MOLT-4, MJ, RPMI-8226, HeLa, KG-1, HEK293T, and SUP-M2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (A700-035-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH (A300-639A).

Detection of human VISTA by western blot

Detection of human VISTA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (A700-035-T lot 1) used for IP at 20 µl per reaction. VISTA was also immunoprecipitated by rabbit anti-VISTA recombinant monoclonal antibody (BL435-5G11). For blotting immunoprecipitated VISTA, A700-035-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.