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  3. A700-035-T
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Rabbit anti-VISTA Recombinant Monoclonal Antibody [BLR035F]

Catalogue number:
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A700-035-T
Supplier:
Bethyl Laboratories, Inc.
Size:
10 µl (5+ tests) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00
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Estimated delivery Fri 3 May
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Applications:

WB, IP, IHC, ICC, IHC-IF, Flow Cyt

Antibody Host:

Rabbit

Target:

VISTA

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

between 250 and 311 (C-term)

Alternative Names:

PP2135; V-set immunoregulatory receptor; V-set domain-containing immunoregulatory receptor; VISTA; sisp-1; stress-induced secreted protein-1; V-type immunoglobulin domain-containing suppressor of T-cell activation; SISP1; B7-H5; platelet receptor GI24; B7

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Product Description:

Rabbit anti-VISTA Recombinant Monoclonal Antibody [BLR035F] - 10 µl (5+ tests)

Storage Temperature:

2 - 8°C

Detection of human VISTA by immunocytoch
Detection of human VISTA by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (A700-035-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human VISTA by immunohistoc
Detection of human VISTA by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (A700-035-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human VISTA by western blot
Detection of human VISTA by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, MOLT-4, MJ, RPMI-8226, HeLa, KG-1, HEK293T, and SUP-M2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (A700-035-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH (A300-639A).
Detection of human VISTA by western blot
Detection of human VISTA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (A700-035-T lot 1) used for IP at 20 µl per reaction. VISTA was also immunoprecipitated by rabbit anti-VISTA recombinant monoclonal antibody (BL435-5G11). For blotting immunoprecipitated VISTA, A700-035-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
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