Rabbit anti-VISTA Recombinant Monoclonal Antibody [BLR035F]
Recombinant antibody was purified from cell culture supernatant.
- Data sheet/protocol: View or download
- MSDS: View or download
- Applications: WB, IP, IHC, ICC, IHC-IF
Antibodies
B7H5, B7-H5, C10orf54, DD1alpha, Death Domain1alpha, GI24, PD-1H, PDCD1 homolog, platelet receptor GI24, PP2135, SISP1, sisp-1, stress-induced secreted protein-1, V-domain Ig suppressor of T cell activation, VISTA, V-set domain-containing immunoregulatory
Human
between 250 and 311 (C-term)
2 - 8°C
Rabbit
Whole IgG
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Recombinant Monoclonal
12352203
Detection of human VISTA by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, MOLT-4, MJ, RPMI-8226, HeLa, KG-1, HEK293T, and SUP-M2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (A700-035-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH (A300-639A).
Detection of human VISTA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-VISTA recombinant monoclonal antibody [BLR035F] (A700-035-T lot 1) used for IP at 20 µl per reaction. VISTA was also immunoprecipitated by rabbit anti-VISTA recombinant monoclonal antibody (BL435-5G11). For blotting immunoprecipitated VISTA, A700-035-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.